PMID- 23512738
OWN - NLM
STAT- MEDLINE
DCOM- 20131113
LR  - 20211021
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Print)
IS  - 1932-8451 (Linking)
VI  - 73
IP  - 8
DP  - 2013 Aug
TI  - Androgen action at the target musculature regulates brain-derived neurotrophic 
      factor protein in the spinal nucleus of the bulbocavernosus.
PG  - 587-98
LID - 10.1002/dneu.22083 [doi]
AB  - We have previously demonstrated that brain-derived neurotrophic factor (BDNF) 
      interacts with testosterone to regulate dendritic morphology of motoneurons in 
      the highly androgen-sensitive spinal nucleus of the bulbocavernosus (SNB). 
      Additionally, in adult male rats testosterone regulates BDNF in SNB motoneurons 
      and its target muscle, the bulbocavernosus (BC). Because BDNF is retrogradely 
      transported from skeletal muscles to spinal motoneurons, we hypothesized that 
      testosterone could regulate BDNF in SNB motoneurons by acting locally at the BC 
      muscle. To test this hypothesis, we restricted androgen manipulation to the SNB 
      target musculature. After castration, BDNF immunolabeling in SNB motoneurons was 
      maintained at levels similar to those of gonadally intact males by delivering 
      testosterone treatment directly to the BC muscle. When the same implant was 
      placed interscapularly in castrated males it was ineffective in supporting BDNF 
      immunolabeling in SNB motoneurons. Furthermore, BDNF immunolabeling in gonadally 
      intact adult males given the androgen receptor blocker hydroxyflutamide delivered 
      directly to the BC muscle was decreased compared with that of gonadally intact 
      animals that had the same hydroxyflutamide implant placed interscapularly, or 
      when compared with castrated animals that had testosterone implants at the 
      muscle. These results demonstrate that the BC musculature is a critical site of 
      action for the androgenic regulation of BDNF in SNB motoneurons and that it is 
      both necessary and sufficient for this action. Furthermore, the local action of 
      androgens at the BC muscle in regulating BDNF provides a possible mechanism 
      underlying the interactive effects of testosterone and BDNF on motoneuron 
      morphology. (c) 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 587-598, 2013.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Verhovshek, Tom
AU  - Verhovshek T
AD  - Department of Neurological Surgery, Indiana Spinal Cord and Brain Injury Research 
      Group, Stark Neurosciences Research Institute, Indiana University School of 
      Medicine, Indianapolis, Indiana, 46202, USA. tverhovs@indiana.edu
FAU - Sengelaub, Dale R
AU  - Sengelaub DR
LA  - eng
GR  - R01 HD035315/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20130624
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (Androgens)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, Androgen)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Androgens/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Castration/adverse effects
MH  - Male
MH  - Motor Neurons/drug effects/*metabolism
MH  - Muscle, Skeletal/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Androgen
MH  - Spinal Cord/*metabolism
MH  - Testosterone/metabolism
PMC - PMC4030717
MID - NIHMS578874
OTO - NOTNLM
OT  - SNB
OT  - bulbocavernosus
OT  - neurotrophic factors
OT  - testosterone
EDAT- 2013/03/21 06:00
MHDA- 2013/11/14 06:00
PMCR- 2014/08/01
CRDT- 2013/03/21 06:00
PHST- 2012/12/20 00:00 [received]
PHST- 2013/02/22 00:00 [revised]
PHST- 2013/03/12 00:00 [accepted]
PHST- 2013/03/21 06:00 [entrez]
PHST- 2013/03/21 06:00 [pubmed]
PHST- 2013/11/14 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1002/dneu.22083 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2013 Aug;73(8):587-98. doi: 10.1002/dneu.22083. Epub 2013 Jun 24.