PMID- 23514766
OWN - NLM
STAT- MEDLINE
DCOM- 20130618
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 143
IP  - 5
DP  - 2013 May
TI  - Resveratrol inhibits IgE-mediated basophilic mast cell degranulation and passive 
      cutaneous anaphylaxis in mice.
PG  - 632-9
LID - 10.3945/jn.112.173302 [doi]
AB  - Resveratrol is a phytoalexin abundantly found in red grape skin and is effective 
      in antitumor and antiinflammation associated with immune responses. This study 
      investigated whether resveratrol suppressed immunoglobulin (Ig)E-mediated 
      allergic responses and passive cutaneous anaphylaxis (PCA) in rat RBL-2H3 mast 
      cells and in BALB/c mice. The release of beta-hexosaminidase and histamine was 
      enhanced in mast cells sensitized with anti-dinitrophenyl (DNP)-IgE and 
      subsequently stimulated by DNP-human serum albumin (HSA), indicative of mast cell 
      degranulation. When mast cells were pretreated with nontoxic resveratrol at 1-25 
      mumol/L, such induction was dose dependently diminished. Spleen tyrosine kinase 
      (Syk) and phospholipase Cgamma (PLCgamma) of sensitized mast cells were activated by 
      stimulation with DNP-HSA antigen, which was dampened by >/=5 mumol/L resveratrol. 
      The phosphorylation of protein kinase C (PKC)mu and PKCtheta was attenuated by 
      administering resveratrol to DNP-HSA-exposed mast cells, whereas quiescent PKCzeta/lambda 
      in sensitized cells was dose-dependently activated by resveratrol. Male BALB/c 
      mice were sensitized for 24 h with DNP-IgE and orally administered with 
      resveratrol 1 h before the DNP-HSA challenge. The histamine concentration was 
      enhanced in sensitized mice challenged to DNP-HSA, which was reversed by 
      administration of 10 mg/kg resveratrol. Additionally, it encumbered the tissue 
      activation of Syk, PLCgamma, and PKCmu in antigen-exposed mice. Resveratrol decreased 
      IgE-mediated PCA and alleviated allergic edema of mouse ear and dorsal skin. Mast 
      cell degranulation and allergic inflammation, accompanying the induction of 
      monocyte chemotactic protein-1 and macrophage inflammatory protein-2, were 
      inhibited by supplementing resveratrol to antigen-challenged mice. Resveratrol 
      inhibited mast cell-derived, immediate-type allergic reactions, and these 
      responses of resveratrol suggest possible therapeutic strategies in preventing 
      allergic inflammatory diseases.
FAU - Han, Seon-Young
AU  - Han SY
AD  - Department of Food Science and Nutrition, Hallym University, Chuncheon, Korea.
FAU - Bae, Ji-Young
AU  - Bae JY
FAU - Park, Sin-Hye
AU  - Park SH
FAU - Kim, Yun-Ho
AU  - Kim YH
FAU - Park, Jung Han Yoon
AU  - Park JH
FAU - Kang, Young-Hee
AU  - Kang YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130320
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (CD24 Antigen)
RN  - 0 (Cd24a protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Dinitrophenols)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Plant Extracts)
RN  - 0 (Serum Albumin)
RN  - 0 (Stilbenes)
RN  - 0 (dinitrophenyl-human serum albumin conjugate)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
RN  - EC 2.7.10.2 (Syk protein, rat)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Anaphylaxis/immunology/metabolism/*prevention & control
MH  - Animals
MH  - Basophils
MH  - CD24 Antigen/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CXCL2/metabolism
MH  - Dietary Supplements
MH  - Dinitrophenols
MH  - Dose-Response Relationship, Drug
MH  - Edema/immunology/prevention & control
MH  - Histamine/metabolism
MH  - Histamine Release/*drug effects
MH  - Immunoglobulin E/*metabolism
MH  - Inflammation/prevention & control
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Male
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Passive Cutaneous Anaphylaxis
MH  - Phospholipase C gamma/metabolism
MH  - Phosphorylation
MH  - *Phytotherapy
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Rats
MH  - Resveratrol
MH  - Serum Albumin
MH  - Skin/*drug effects/immunology/metabolism
MH  - Stilbenes/pharmacology/*therapeutic use
MH  - Syk Kinase
MH  - Vitis/chemistry
MH  - beta-N-Acetylhexosaminidases/metabolism
EDAT- 2013/03/22 06:00
MHDA- 2013/06/19 06:00
CRDT- 2013/03/22 06:00
PHST- 2013/03/22 06:00 [entrez]
PHST- 2013/03/22 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - S0022-3166(22)01188-9 [pii]
AID - 10.3945/jn.112.173302 [doi]
PST - ppublish
SO  - J Nutr. 2013 May;143(5):632-9. doi: 10.3945/jn.112.173302. Epub 2013 Mar 20.