PMID- 2351740
OWN - NLM
STAT- MEDLINE
DCOM- 19900717
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 5
DP  - 1990 May
TI  - Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA 
      in peripheral blood of patients with exanthem subitum.
PG  - 970-4
AB  - The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA 
      in peripheral blood mononuclear cells from patients with exanthem subitum. 
      Amplified products were detected by agarose gel electrophoresis and dot blot 
      hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five 
      other human herpesviruses was observed in this system, and all HHV-6 strains gave 
      positive reactions when the primer pairs were used. Immunoglobulin M antibody 
      appeared about 5 days after the onset of disease, reaching a maximum after about 
      2 to 3 weeks and then decreasing to less than 1:10 1 month after the onset of 
      disease in almost all patients. Mononuclear cells from seven patients with 
      exanthem subitum all gave positive reactions in this system, and viral DNA was 
      found in samples even during the convalescent phase of the disease. We conclude 
      that this test, using polymerase chain reaction amplification, which takes only 1 
      to 2 days, is useful for the diagnosis of HHV-6 infection.
FAU - Kondo, K
AU  - Kondo K
AD  - Department of Virology, Osaka University, Japan.
FAU - Hayakawa, Y
AU  - Hayakawa Y
FAU - Mori, H
AU  - Mori H
FAU - Sato, S
AU  - Sato S
FAU - Kondo, T
AU  - Kondo T
FAU - Takahashi, K
AU  - Takahashi K
FAU - Minamishima, Y
AU  - Minamishima Y
FAU - Takahashi, M
AU  - Takahashi M
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Antibodies, Viral/blood
MH  - DNA, Viral/*blood
MH  - Exanthema Subitum/blood/immunology/*microbiology
MH  - *Gene Amplification
MH  - Herpesviridae/classification/immunology/*isolation & purification
MH  - Humans
MH  - Leukocytes, Mononuclear/microbiology
MH  - *Polymerase Chain Reaction
MH  - Species Specificity
MH  - Time Factors
PMC - PMC267848
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
PMCR- 1990/05/01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
PHST- 1990/05/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.5.970-974.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 May;28(5):970-4. doi: 10.1128/jcm.28.5.970-974.1990.