PMID- 23517468
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20171116
IS  - 1939-6376 (Electronic)
IS  - 1939-6368 (Linking)
VI  - 59
IP  - 4
DP  - 2013 Aug
TI  - Contraindication of ART following a sperm FISH analysis, even though only 12% of 
      the spermatozoa had enlarged heads.
PG  - 214-7
LID - 10.3109/19396368.2013.783646 [doi]
AB  - We report on a couple with a five-year history of idiopathic primary infertility. 
      Two early miscarriages had followed intrauterine insemination (IUI). The man's 
      fertility was then re-evaluated, in order to establish whether or not IUI was the 
      best treatment option. Although the semen parameters were normal (sperm 
      concentration: 89 million/ml; progressive motility: 40%; percentage of typical 
      forms: 20%), a computer-assisted sperm morphology analysis with strict criteria 
      found that 12% of the spermatozoa had enlarged heads. All of the latter had a 
      normal form and none had multiple flagella. Using fluorescence in situ 
      hybridization (FISH) analysis, we found that the proportion of aneuploid and 
      diploid spermatozoa was 78% for the sample as a whole and 68% for normally-shaped 
      spermatozoa with a normal-sized head. Although treatment options are well 
      documented for men with macrocephalic sperm head syndrom, there is no consensus 
      on individuals with a low but non-negligible proportion of spermatozoa with 
      enlarged heads. Here, our FISH results contraindicated the use of assisted 
      reproductive technology with the man's sperm. The couple decided to resort to 
      donor sperm.
FAU - Guthauser, Bruno
AU  - Guthauser B
AD  - Department of Reproductive Biology, Dreux Hospital, Dreux, France. 
      brunoguthauser@gmail.com
FAU - Boitrelle, Florence
AU  - Boitrelle F
FAU - Albert, Martine
AU  - Albert M
FAU - Ketata, Fadoua
AU  - Ketata F
FAU - Meynant, Celine
AU  - Meynant C
FAU - Ferfouri, Fatma
AU  - Ferfouri F
FAU - Selva, Jacqueline
AU  - Selva J
FAU - Vialard, Francois
AU  - Vialard F
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20130321
PL  - England
TA  - Syst Biol Reprod Med
JT  - Systems biology in reproductive medicine
JID - 101464963
SB  - IM
MH  - Aneuploidy
MH  - Contraindications
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/genetics/*pathology
MH  - Male
MH  - *Reproductive Techniques, Assisted
MH  - Semen Analysis
MH  - Sperm Head/*pathology
EDAT- 2013/03/23 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/03/23 06:00
PHST- 2013/03/23 06:00 [entrez]
PHST- 2013/03/23 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - 10.3109/19396368.2013.783646 [doi]
PST - ppublish
SO  - Syst Biol Reprod Med. 2013 Aug;59(4):214-7. doi: 10.3109/19396368.2013.783646. 
      Epub 2013 Mar 21.