PMID- 23518306
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20130429
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 80
DP  - 2013 Jun
TI  - Qualitative and quantitative analysis of the major constituents in Chinese 
      medicinal preparation Dan-Lou tablet by ultra high performance liquid 
      chromatography/diode-array detector/quadrupole time-of-flight tandem mass 
      spectrometry.
PG  - 50-62
LID - S0731-7085(13)00071-X [pii]
LID - 10.1016/j.jpba.2013.02.011 [doi]
AB  - A rapid ultra high performance liquid chromatography/diode-array 
      detector/quadrupole time-of-flight tandem mass spectrometry (UPLC-DAD-QTOF) 
      method and a ultra high performance liquid chromatography coupled with 
      diode-array detector (UPLC-DAD) method were developed for qualitative and 
      quantitative analyses of the major chemical constituents in Dan-Lou tablet. 
      Sixty-eight compounds including flavonoids, phenolic acids, tanshinones, 
      protostane triterpenoids, lactones, and paeoniflorins were unambiguously or 
      tentatively identified by comparing their retention times and accurate mass 
      measurement in 40min with references or literature data. Among them, 19 
      compounds: gallic acid, danshensu, 5-hydroxymethyl-2-furaldehyde, 3'-hydroxy 
      puerarin, puerarin, 3'-methoxy puerarin, mirificin, daidzin, paeoniflorin, 
      calycosin-7-O-beta-D-glucoside, naringin, genistin, rosmarinic acid, salvianolic 
      acid B, salvianolic acid A, formononetin, calycosin, cryptotanshinone and 
      tanshinone IIA were further quantified in 30min as marker substances. It was 
      found that the calibration curves for all analytes showed good linearity 
      (R(2)>0.9997) within the test ranges. The overall limits of detection (LODs) and 
      limits of quantification (LOQs) were 0.0073-0.34mug/mL and 0.022-1.04mug/mL, 
      respectively. The relative standard deviations (RSDs) for intra- and inter-day 
      precisions were below 1.90% and 2.85%, respectively. The results of repeatability 
      were less than 2.74%. The sample was stable for at least 48h. The mean recovery 
      rates ranged from 95.5% to 105% with the relative standard deviations (RSDs) less 
      than 2.96%. The results showed that the developed quantitative method was linear, 
      sensitive, and precise for quality control of Dan-Lou tablet.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Dong, Juan
AU  - Dong J
AD  - Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin 300193, PR China.
FAU - Zhu, Yan
AU  - Zhu Y
FAU - Gao, Xiumei
AU  - Gao X
FAU - Chang, Yanxu
AU  - Chang Y
FAU - Wang, Meng
AU  - Wang M
FAU - Zhang, Peng
AU  - Zhang P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130227
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Tablets)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*analysis/chemistry
MH  - Limit of Detection
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tablets
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/03/23 06:00
MHDA- 2013/11/13 06:00
CRDT- 2013/03/23 06:00
PHST- 2012/10/26 00:00 [received]
PHST- 2013/02/11 00:00 [revised]
PHST- 2013/02/13 00:00 [accepted]
PHST- 2013/03/23 06:00 [entrez]
PHST- 2013/03/23 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
AID - S0731-7085(13)00071-X [pii]
AID - 10.1016/j.jpba.2013.02.011 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2013 Jun;80:50-62. doi: 10.1016/j.jpba.2013.02.011. Epub 
      2013 Feb 27.