PMID- 23519994
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20220408
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 28
IP  - 9
DP  - 2013 Sep
TI  - Monocyte chemoattractant protein-1 is a mediator of the anabolic action of 
      parathyroid hormone on bone.
PG  - 1975-86
LID - 10.1002/jbmr.1933 [doi]
AB  - Parathyroid hormone (PTH) has a significant role as an anabolic hormone in bone 
      when administered by intermittent injection. Previous microarray studies in our 
      laboratory have shown that the most highly regulated gene, monocyte 
      chemoattractant protein-1 (MCP-1), is rapidly and transiently induced when 
      hPTH(1-34) is injected intermittently in rats. Through further in vivo studies, 
      we found that rats treated with hPTH(1-34) showed a significant increase in serum 
      MCP-1 levels 2 hours after PTH injection compared with basal levels. Using 
      immunohistochemistry, increased MCP-1 expression in osteoblasts and osteocytes is 
      evident after PTH treatment. PTH also increased the number of marrow macrophages. 
      MCP-1 knockout mice injected daily with hPTH(1-34) showed less trabecular bone 
      mineral density and bone volume compared with wild-type mice as measured by 
      peripheral quantitative computed tomography (pQCT) and micro-computed tomography 
      (microCT). Histomorphometric analysis revealed that the increase in osteoclast 
      surface and osteoclast number observed with intermittent PTH treatment in the 
      wild-type mice was completely eliminated in the MCP-1 null mice, as well as much 
      lower numbers of macrophages. Consequently, the lack of osteoclast and macrophage 
      activity in the MCP-1 null mice was paralleled by a reduction in bone formation. 
      We conclude that osteoblast and osteocyte MCP-1 expression is an important 
      mediator for the anabolic effects of PTH on bone.
CI  - Copyright (c) 2013 American Society for Bone and Mineral Research.
FAU - Tamasi, Joseph A
AU  - Tamasi JA
AD  - Department of Physiology and Biophysics, University of Medicine and Dentistry of 
      New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ, USA.
FAU - Vasilov, Anatoliy
AU  - Vasilov A
FAU - Shimizu, Emi
AU  - Shimizu E
FAU - Benton, Noah
AU  - Benton N
FAU - Johnson, Joshua
AU  - Johnson J
FAU - Bitel, Claudine L
AU  - Bitel CL
FAU - Morrison, Nigel
AU  - Morrison N
FAU - Partridge, Nicola C
AU  - Partridge NC
LA  - eng
GR  - R01 DK047420/DK/NIDDK NIH HHS/United States
GR  - R01 DK048109/DK/NIDDK NIH HHS/United States
GR  - R56 DK047420/DK/NIDDK NIH HHS/United States
GR  - DK48109/DK/NIDDK NIH HHS/United States
GR  - DK47420/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Anabolic Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Parathyroid Hormone)
SB  - IM
MH  - Anabolic Agents/*pharmacology
MH  - Animals
MH  - Bone Density/drug effects
MH  - Bone and Bones/cytology/diagnostic imaging/*drug effects/*metabolism
MH  - Chemokine CCL2/blood/deficiency/*metabolism
MH  - Female
MH  - Femur/cytology/diagnostic imaging/drug effects/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Organ Size/drug effects
MH  - Osteogenesis/drug effects
MH  - Parathyroid Hormone/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tibia/cytology/diagnostic imaging/drug effects/metabolism
MH  - X-Ray Microtomography
PMC - PMC3749291
MID - NIHMS457124
OTO - NOTNLM
OT  - BONE ANABOLISM
OT  - BONE TURNOVER
OT  - CYTOKINES
OT  - MONOCYTE CHEMOATTRACTANT PROTEIN (MCP-1)
OT  - PARATHYROID HORMONE (PTH)
COIS- Conflicts of Interest The authors state that they have no conflicts of interest 
      pertaining to the work described.
EDAT- 2013/03/23 06:00
MHDA- 2014/03/07 06:00
PMCR- 2014/09/01
CRDT- 2013/03/23 06:00
PHST- 2012/07/11 00:00 [received]
PHST- 2013/02/27 00:00 [revised]
PHST- 2013/03/12 00:00 [accepted]
PHST- 2013/03/23 06:00 [entrez]
PHST- 2013/03/23 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.1002/jbmr.1933 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2013 Sep;28(9):1975-86. doi: 10.1002/jbmr.1933.