PMID- 23521165
OWN - NLM
STAT- MEDLINE
DCOM- 20130719
LR  - 20131121
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 125
IP  - 5
DP  - 2013 Jun
TI  - Memory impairment and hippocampus specific protein oxidation induced by ethanol 
      intake and 3, 4-methylenedioxymethamphetamine (MDMA) in mice.
PG  - 736-46
LID - 10.1111/jnc.12247 [doi]
AB  - Ethanol and 3, 4-Methylenedioxymethamphetamine (MDMA) are popular recreational 
      drugs widely abused by adolescents that may induce neurotoxic processes 
      associated with behavioural alterations. Adolescent CD1 mice were subjected to 
      ethanol intake using the drinking in the dark (DID) procedure, acute MDMA or a 
      combination. Considering that both drugs of abuse cause oxidative stress in the 
      brain, protein oxidative damage in different brain areas was analysed 72 h after 
      treatment using a proteomic approach. Damage to specific proteins in treated 
      animals was significant in the hippocampus but not in the prefrontal cortex. The 
      damaged hippocampus proteins were then identified by mass spectrometry, revealing 
      their involvement in energy metabolism, structural function, axonal outgrowth and 
      stability, and neurotransmitter release. Mice treated with MDMA displayed higher 
      oxidative damage than ethanol-treated mice. To determine whether this oxidative 
      damage was affecting hippocampus activity, declarative memory was evaluated at 
      72 h after treatment using the object recognition assay and the radial arm maze. 
      Although acquisition in the radial arm maze was not impaired by ethanol intake, 
      MDMA treatment impaired long-term memory in both tests. Therefore, oxidative 
      damage to specific proteins observed under MDMA treatment affects important 
      cellular function on the hippocampus that may contribute to declarative memory 
      deficits.
CI  - (c) 2013 International Society for Neurochemistry.
FAU - Ros-Simo, Clara
AU  - Ros-Simo C
AD  - Grup de Recerca en Neurobiologia del Comportament GReNeC, Departament de Ciencies 
      Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomedica 
      de Barcelona, 08003 Barcelona, Spain.
FAU - Moscoso-Castro, Maria
AU  - Moscoso-Castro M
FAU - Ruiz-Medina, Jessica
AU  - Ruiz-Medina J
FAU - Ros, Joaquim
AU  - Ros J
FAU - Valverde, Olga
AU  - Valverde O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130422
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Membrane Proteins)
RN  - 3K9958V90M (Ethanol)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Ethanol/*toxicity
MH  - Hippocampus/*drug effects/metabolism
MH  - Male
MH  - Maze Learning/drug effects/physiology
MH  - Membrane Proteins/metabolism
MH  - Memory Disorders/*chemically induced/metabolism
MH  - Mice
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*toxicity
MH  - Oxidation-Reduction/drug effects
MH  - Oxidative Stress/*drug effects/physiology
EDAT- 2013/03/26 06:00
MHDA- 2013/07/20 06:00
CRDT- 2013/03/26 06:00
PHST- 2013/01/14 00:00 [received]
PHST- 2013/02/20 00:00 [revised]
PHST- 2013/03/21 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2013/07/20 06:00 [medline]
AID - 10.1111/jnc.12247 [doi]
PST - ppublish
SO  - J Neurochem. 2013 Jun;125(5):736-46. doi: 10.1111/jnc.12247. Epub 2013 Apr 22.