PMID- 23521775
OWN - NLM
STAT- MEDLINE
DCOM- 20140314
LR  - 20161125
IS  - 1469-5111 (Electronic)
IS  - 1461-1457 (Linking)
VI  - 16
IP  - 8
DP  - 2013 Sep
TI  - Involvement of prelimbic medial prefrontal cortex in panic-like elaborated 
      defensive behaviour and innate fear-induced antinociception elicited by GABAA 
      receptor blockade in the dorsomedial and ventromedial hypothalamic nuclei: role 
      of the endocannabinoid CB1 receptor.
PG  - 1781-98
LID - 10.1017/S1461145713000163 [doi]
AB  - It has been shown that GABAA receptor blockade in the dorsomedial and 
      ventromedial hypothalamic nuclei (DMH and VMH, respectively) induces elaborated 
      defensive behavioural responses accompanied by antinociception, which has been 
      utilized as an experimental model of panic attack. Furthermore, the prelimbic 
      (PL) division of the medial prefrontal cortex (MPFC) has been related to 
      emotional reactions and the processing of nociceptive information. The aim of the 
      present study was to investigate the possible involvement of the PL cortex and 
      the participation of local cannabinoid CB1 receptors in the elaboration of 
      panic-like reactions and in innate fear-induced antinociception. Elaborated 
      fear-induced responses were analysed during a 10-min period in an open-field test 
      arena. Microinjection of the GABAA receptor antagonist bicuculline into the 
      DMH/VMH evoked panic-like behaviour and fear-induced antinociception, which was 
      decreased by microinjection of the non-selective synaptic contact blocker cobalt 
      chloride in the PL cortex. Moreover, microinjection of AM251 (25, 100 or 
      400 pmol), an endocannabinoid CB1 receptor antagonist, into the PL cortex also 
      attenuated the defensive behavioural responses and the antinociception that 
      follows innate fear behaviour elaborated by DMH/VMH. These data suggest that the 
      PL cortex plays an important role in the organization of elaborated forward 
      escape behaviour and that this cortical area is also involved in the elaboration 
      of innate fear-induced antinociception. Additionally, CB1 receptors in the PL 
      cortex modulate both panic-like behaviours and fear-induced antinociception 
      elicited by disinhibition of the DMH/VMH through microinjection of bicuculline.
FAU - Freitas, Renato Leonardo de
AU  - Freitas RL
AD  - Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, 
      Ribeirao Preto Medical School of the University of Sao Paulo, Av. dos 
      Bandeirantes 3900, Ribeirao Preto, SP, Brazil.
FAU - Salgado-Rohner, Carlos Jose
AU  - Salgado-Rohner CJ
FAU - Hallak, Jaime Eduardo Cecilio
AU  - Hallak JE
FAU - Crippa, Jose Alexandre de Souza
AU  - Crippa JA
FAU - Coimbra, Norberto Cysne
AU  - Coimbra NC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130325
PL  - England
TA  - Int J Neuropsychopharmacol
JT  - The international journal of neuropsychopharmacology
JID - 9815893
RN  - 0 (GABA Antagonists)
RN  - 0 (Piperidines)
RN  - 0 (Pyrazoles)
RN  - 0 (Receptor, Cannabinoid, CB1)
RN  - 0 (Receptors, GABA-A)
RN  - 3I4FA44MAI (AM 251)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Bicuculline/pharmacology/therapeutic use
MH  - *Defense Mechanisms
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - GABA Antagonists/pharmacology/therapeutic use
MH  - Hyperalgesia/drug therapy/pathology
MH  - Hypothalamus/*drug effects/physiology
MH  - Instinct
MH  - Male
MH  - Microinjections
MH  - Pain Measurement/*drug effects
MH  - Pain Threshold/drug effects
MH  - Panic/*drug effects
MH  - Piperidines/pharmacology
MH  - Prefrontal Cortex/drug effects/*physiopathology
MH  - Pyrazoles/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Cannabinoid, CB1/antagonists & inhibitors/*metabolism
MH  - Receptors, GABA-A/*metabolism
EDAT- 2013/03/26 06:00
MHDA- 2014/03/15 06:00
CRDT- 2013/03/26 06:00
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2014/03/15 06:00 [medline]
AID - S1461145713000163 [pii]
AID - 10.1017/S1461145713000163 [doi]
PST - ppublish
SO  - Int J Neuropsychopharmacol. 2013 Sep;16(8):1781-98. doi: 
      10.1017/S1461145713000163. Epub 2013 Mar 25.