PMID- 23522536
OWN - NLM
STAT- MEDLINE
DCOM- 20140225
LR  - 20130325
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 39
IP  - 4
DP  - 2013 Apr
TI  - Elevated tumor necrosis factor-alpha expression in periapical lesions infected by 
      Epstein-Barr virus.
PG  - 456-60
LID - S0099-2399(13)00018-6 [pii]
LID - 10.1016/j.joen.2012.12.028 [doi]
AB  - INTRODUCTION: In apical periodontitis, there is an intense inflammatory response 
      to endodontopathogenic bacteria, an essential component of the pathogenic 
      microbiota. The inflammation can be aggravated by herpesviruses acting as 
      nonessential pathogens in periapical lesions. This study aimed to determine the 
      levels of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta 
      (TGF-beta) in periapical lesions in relation to local occurrence of Epstein-Barr 
      virus (EBV), human cytomegalovirus (HCMV), human herpesvirus 6 (HHV-6), and human 
      herpesvirus 8 (HHV-8). METHODS: Fifty-eight samples with apical periodontitis and 
      20 clinically healthy gingival control tissues were collected. Viral DNA was 
      determined with nested polymerase chain reaction, and cytokine mRNA expression 
      was detected with real-time polymerase chain reaction assays. RESULTS: Periapical 
      lesions harbored EBV (75.9%) and HHV-6 (22.4%) at significantly higher 
      frequencies compared with controls (P < .000001 and P < .05, respectively), 
      whereas HCMV (12%) and HHV-8 (0%) occurred rarely. The median TNF-alpha expression 
      was 13 times higher (P < .001) and TGF-beta expression was 5 times higher in 
      periapical lesions than in controls (P < .001). TNF-alpha expression was 
      significantly higher in EBV-positive lesions than in EBV-negative lesions (P = 
      .032). Presence of symptoms, lesion size, and infection by HCMV or HHV-6 had no 
      significant association with either TNF-alpha or TGF-beta expression. CONCLUSIONS: The 
      herpesviral component of the endodontic microbiota did not correlate with TGF-beta 
      expression, whereas EBV infection was associated with a median 1.5 times further 
      elevation of the high TNF-alpha expression characteristic for periapical lesions.
CI  - Copyright (c) 2013 American Association of Endodontists. Published by Elsevier Inc. 
      All rights reserved.
FAU - Hernadi, Katinka
AU  - Hernadi K
AD  - Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
FAU - Gyongyosi, Eszter
AU  - Gyongyosi E
FAU - Meszaros, Beata
AU  - Meszaros B
FAU - Szakacs, Levente
AU  - Szakacs L
FAU - Szalmas, Anita
AU  - Szalmas A
FAU - Csoma, Eszter
AU  - Csoma E
FAU - Mogyorosi, Richard
AU  - Mogyorosi R
FAU - Czompa, Levente
AU  - Czompa L
FAU - Veress, Gyorgy
AU  - Veress G
FAU - Varga, Istvan
AU  - Varga I
FAU - Marton, Ildiko Judit
AU  - Marton IJ
FAU - Konya, Jozsef
AU  - Konya J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130210
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (DNA, Viral)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
MH  - Adult
MH  - Case-Control Studies
MH  - Chi-Square Distribution
MH  - Cytomegalovirus
MH  - DNA, Viral/analysis
MH  - Epstein-Barr Virus Infections/*immunology/metabolism
MH  - Herpesvirus 4, Human
MH  - Herpesvirus 6, Human
MH  - Herpesvirus 8, Human
MH  - Humans
MH  - Middle Aged
MH  - Periapical Periodontitis/*immunology/metabolism/pathology/*virology
MH  - Real-Time Polymerase Chain Reaction
MH  - Statistics, Nonparametric
MH  - Transforming Growth Factor beta/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2013/03/26 06:00
MHDA- 2014/02/26 06:00
CRDT- 2013/03/26 06:00
PHST- 2012/09/14 00:00 [received]
PHST- 2012/12/13 00:00 [revised]
PHST- 2012/12/30 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2014/02/26 06:00 [medline]
AID - S0099-2399(13)00018-6 [pii]
AID - 10.1016/j.joen.2012.12.028 [doi]
PST - ppublish
SO  - J Endod. 2013 Apr;39(4):456-60. doi: 10.1016/j.joen.2012.12.028. Epub 2013 Feb 
      10.