PMID- 23523171
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 164
IP  - 3-4
DP  - 2013 Jun 28
TI  - Identification, virulence, and mass spectrometry of toxic ECP fractions of West 
      Alabama isolates of Aeromonas hydrophila obtained from a 2010 disease outbreak.
PG  - 336-43
LID - S0378-1135(13)00134-X [pii]
LID - 10.1016/j.vetmic.2013.02.020 [doi]
AB  - In West Alabama, disease outbreaks in 2009 caused by Aeromonas hydrophila have 
      led to an estimated loss of more than $3 million. In 2010, disease outbreak 
      occurred again in West Alabama, causing losses of hundreds of thousands of pounds 
      of market size channel catfish. During the 2010 disease outbreak in West Alabama, 
      four isolates of A. hydrophila were cultured from the kidney tissues of diseased 
      channel catfish. Both analytical profile index (API) 20 E biochemical tests and 
      16S-23S rRNA sequencing results confirmed the four isolates as A. hydrophila. 
      Virulence studies revealed that the four isolates were highly virulent to channel 
      catfish by intraperitoneal injection, with LD50 value of  approximately  1.3 x 10(5)CFU/fish. 
      Extracellular proteins (ECPs) of A. hydrophila are well known to be toxic to 
      fish. Therefore, ECPs of the four 2010 West Alabama isolates of A. hydrophila 
      were characterized in this study. The ECPs of the four 2010 isolates were found 
      to be toxic to channel catfish fingerlings, with LD50 value of 16 mug/fish. Thirty 
      ECP fractions were obtained from the ECPs of the 2010 isolates of A. hydrophila 
      by cation-exchange chromatography, of which nine fractions were found to be toxic 
      to catfish gill cells and channel catfish fingerlings. Mass spectrometry 
      identified 228 proteins from the nine toxic fractions, of which 23 were shared by 
      toxic fractions, including well known virulence factors such as hemolysin, 
      aerolysin, elastase (metalloprotease), nuclease, and 5'-nucleotidase. Hemolytic 
      activity, protease activity, and nuclease activity of the four isolates were 
      found to be significantly (P<0.05) higher than that of a reference A. hydrophila 
      strain AL98-C1B. Our results might shed light on the possible virulence factors 
      of the highly virulent West Alabama isolates of A. hydrophila.
CI  - Published by Elsevier B.V.
FAU - Pridgeon, Julia W
AU  - Pridgeon JW
AD  - Aquatic Animal Health Research Unit, USDA-ARS, 990 Wire Road, Auburn, AL 36830, 
      USA. Julia.Pridgeon@ars.usda.gov
FAU - Klesius, Phillip H
AU  - Klesius PH
FAU - Song, Lin
AU  - Song L
FAU - Zhang, Dunhua
AU  - Zhang D
FAU - Kojima, Kyoko
AU  - Kojima K
FAU - Mobley, James A
AU  - Mobley JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130228
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 23S)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Aeromonas hydrophila/*chemistry/genetics/isolation & purification/*pathogenicity
MH  - Alabama
MH  - Animals
MH  - Bacterial Proteins/*chemistry/isolation & purification/*metabolism
MH  - Fish Diseases/*microbiology
MH  - Gram-Negative Bacterial Infections/microbiology/*veterinary
MH  - Ictaluridae
MH  - Lethal Dose 50
MH  - Mass Spectrometry
MH  - RNA, Ribosomal, 16S/genetics
MH  - RNA, Ribosomal, 23S/genetics
MH  - Virulence/genetics
MH  - Virulence Factors/chemistry/genetics/metabolism
EDAT- 2013/03/26 06:00
MHDA- 2014/04/09 06:00
CRDT- 2013/03/26 06:00
PHST- 2013/01/18 00:00 [received]
PHST- 2013/02/19 00:00 [revised]
PHST- 2013/02/20 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
AID - S0378-1135(13)00134-X [pii]
AID - 10.1016/j.vetmic.2013.02.020 [doi]
PST - ppublish
SO  - Vet Microbiol. 2013 Jun 28;164(3-4):336-43. doi: 10.1016/j.vetmic.2013.02.020. 
      Epub 2013 Feb 28.