PMID- 23523995
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20181202
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 241
DP  - 2013 Jun 25
TI  - Resveratrol protects spinal cord dorsal column from hypoxic injury by activating 
      Nrf-2.
PG  - 80-8
LID - S0306-4522(13)00230-3 [pii]
LID - 10.1016/j.neuroscience.2013.03.015 [doi]
AB  - Damage from oxidative stress plays a critical role in spinal cord injury. Nuclear 
      factor erythroid 2-related factor (Nrf-2) signaling pathway can be activated by 
      cellular oxidative stress. Resveratrol, a plant-derived polyphenolic compound 
      found in red wine, has antioxidant properties. In the present study, we have 
      examined the neuroprotective effect of resveratrol and the role of Nrf-2 in 
      spinal cord hypoxic injury. The spinal cord was removed from adult male Wistar 
      rats from T2-T10 and the dorsal column was used to induce hypoxic injury in vitro 
      with and without treatment with resveratrol (50muM). Significant changes were 
      found in the compound action potential (CAP) of spinal cord dorsal column, and 
      hematoxyline and eosin (H&E) staining showed that resveratrol significantly 
      improved neuronal injury. The biochemical assays showed significant changes in 
      lipid peroxidase (LPO), reduced glutathione (GSH), superoxide dismutase (SOD), 
      protein carbonyl (PC), mitochondrial ATP content, and mitochondrial Ca(++). 
      Furthermore, using immunohistochemistry and Western blot, we found that after 
      resveratrol treatment during hypoxic injury there was a significant activation of 
      NrF-2 and down regulation of the glial fibrillary acidic protein (GFAP) content. 
      The results show that resveratrol treatment has neuroprotective effects on CAP, 
      Ca(++) loading, and biochemical parameters after hypoxic injury. The 
      neuroprotective effect is likely to be exerted by increased activation of 
      transcription factor Nrf-2 by resveratrol along with its direct antioxidant 
      effect to ameliorate the oxidative damage and preserve mitochondrial function.
CI  - Published by Elsevier Ltd.
FAU - Kesherwani, V
AU  - Kesherwani V
AD  - Division of Neurosurgery, Department of Surgery, University of Nebraska Medical 
      Center, Omaha, NE 68198-7690, USA.
FAU - Atif, F
AU  - Atif F
FAU - Yousuf, S
AU  - Yousuf S
FAU - Agrawal, S K
AU  - Agrawal SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130320
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Stilbenes)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Blotting, Western
MH  - Fluorescent Antibody Technique
MH  - Hypoxia/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Resveratrol
MH  - Spinal Cord/*drug effects
MH  - Spinal Cord Injuries/*metabolism
MH  - Stilbenes/*pharmacology
EDAT- 2013/03/26 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/03/26 06:00
PHST- 2012/12/13 00:00 [received]
PHST- 2013/02/12 00:00 [revised]
PHST- 2013/03/08 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - S0306-4522(13)00230-3 [pii]
AID - 10.1016/j.neuroscience.2013.03.015 [doi]
PST - ppublish
SO  - Neuroscience. 2013 Jun 25;241:80-8. doi: 10.1016/j.neuroscience.2013.03.015. Epub 
      2013 Mar 20.