PMID- 23524252
OWN - NLM
STAT- MEDLINE
DCOM- 20140428
LR  - 20181202
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 27
IP  - 3-4
DP  - 2013 Jun-Aug
TI  - Multiplex PCR for the simultaneous detection of porcine reproductive and 
      respiratory syndrome virus, classical swine fever virus, and porcine circovirus 
      in pigs.
PG  - 149-52
LID - S0890-8508(13)00015-7 [pii]
LID - 10.1016/j.mcp.2013.03.001 [doi]
AB  - A multiplex polymerase chain reaction (PCR) was designed for the simultaneous 
      detection of three viruses involved in reproductive and respiratory failure in 
      pigs: porcine reproductive and respiratory syndrome virus (PRRSV), classical 
      swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2). Each target 
      produced a specific amplicon with a size of 718 bp (PRRSV), 288 bp (CSFV), or 466 
      bp (PCV-2). The sensitivity of the multiplex PCR using purified plasmid 
      constructs containing the specific viral target fragments was 2.0 x 10(4), 
      2.5 x 10(3), and 6.0 x 10(2) copies for PRRSV, CSFV, and PCV-2, respectively. 
      Non-specific reactions were not observed when other viruses, bacteria, and 
      PK-15/Marc-145 cells were used to assess the multiplex PCR. Among 82 clinical 
      samples from Fujian province, co-infection by PRRSV and CSFV was 12.19%, 
      co-infection by PRRSV and PCV-2 was 21.95%, CSFV and PCV-2 was 13.41%, and 
      co-infection by the three viruses was 3.66%. In conclusion, the multiplex PCR 
      should be useful for routine molecular diagnosis and epidemiology. The multiplex 
      PCR was effective in detecting various combinations of one or more of these 
      viruses in pig specimens.
CI  - Copyright (c) 2013. Published by Elsevier Ltd.
FAU - Liu, Jian-Kui
AU  - Liu JK
AD  - College of Life Sciences of Longyan University, Longyan, Fujian Province 364012, 
      China. liujiankui99@126.com
FAU - Wei, Chun-Hua
AU  - Wei CH
FAU - Yang, Xiao-Yan
AU  - Yang XY
FAU - Dai, Ai-Ling
AU  - Dai AL
FAU - Li, Xiao-Hua
AU  - Li XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130319
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Circovirus/*genetics
MH  - Classical Swine Fever Virus/*genetics
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA, Viral/analysis
MH  - Swine
MH  - Swine Diseases/virology
EDAT- 2013/03/26 06:00
MHDA- 2014/04/29 06:00
CRDT- 2013/03/26 06:00
PHST- 2012/11/03 00:00 [received]
PHST- 2013/03/04 00:00 [revised]
PHST- 2013/03/04 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2014/04/29 06:00 [medline]
AID - S0890-8508(13)00015-7 [pii]
AID - 10.1016/j.mcp.2013.03.001 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2013 Jun-Aug;27(3-4):149-52. doi: 10.1016/j.mcp.2013.03.001. 
      Epub 2013 Mar 19.