PMID- 23524880
OWN - NLM
STAT- MEDLINE
DCOM- 20160127
LR  - 20150225
IS  - 1477-0393 (Electronic)
IS  - 0748-2337 (Linking)
VI  - 31
IP  - 3
DP  - 2015 Mar
TI  - Citreoviridin enhances tumor necrosis factor-alpha-induced adhesion of human 
      umbilical vein endothelial cells.
PG  - 193-201
LID - 10.1177/0748233713483194 [doi]
AB  - Endothelial adhesion plays an important role in the process of atherosclerosis, 
      which is regulated by endothelial adhesion molecules and chemoattractant 
      molecules. In some areas of China, citreoviridin (CIT) is considered a risk 
      factor for the development of atherosclerosis. Here, we investigated the role of 
      CIT in adhesion of human umbilical vein endothelial cells (HUVECs) together with 
      the stimulation of tumor necrosis factor-alpha (TNF-alpha). Adhesion of HUVECs to 
      monocytes was analyzed by coculture experiments using U937 cells labeled with 
      2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethylester. The 
      expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion 
      molecule-1 (VCAM-1), and E-selectin was determined by Western blot and 
      enzyme-linked immunosorbent assay (ELISA). The expression of monocyte 
      chemoattractant protein-1 (MCP-1) was measured by reverse transcription 
      polymerase chain reaction and ELISA. The activation of nuclear factor-kappaB (NF-kappaB) 
      was assessed by Western blot and immunofluorescence staining. CIT markedly 
      increased TNF-alpha-induced HUVECs adhesion to monocytes and the expression levels of 
      ICAM-1, VCAM-1, E-selectin, and MCP-1. TNF-alpha-induced nuclear translocation of 
      NF-kappaB in HUVECs was significantly elevated by CIT. Our study demonstrates that 
      CIT upregulates TNF-alpha-induced endothelial adhesion via increasing activation of 
      NF-kappaB, which results in the expression of ICAM-1, VCAM-1, E-selectin, and MCP-1. 
      CIT plays a pivotal role in the process of endothelial cell adhesion and may 
      thereby play an important role in the improvement of atherosclerosis in areas of 
      China that have a high prevalence of CIT contamination and atherosclerosis.
CI  - (c) The Author(s) 2013.
FAU - Hou, Haifeng
AU  - Hou H
AD  - Institute of Epidemiology, School of Public Health, Shandong University, Jinan, 
      China Institute of Epidemiology, School of Public Health, Taishan Medical 
      University, Taian, China.
FAU - Zhou, Ru
AU  - Zhou R
AD  - Institute of Epidemiology, School of Public Health, Taishan Medical University, 
      Taian, China.
FAU - Jia, Qiang
AU  - Jia Q
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, Beijing, China.
FAU - Li, Qunwei
AU  - Li Q
AD  - Institute of Epidemiology, School of Public Health, Taishan Medical University, 
      Taian, China.
FAU - Kang, Li
AU  - Kang L
AD  - Institute of Atherosclerosis, Taishan Medical University, Taian, China.
FAU - Jiao, Peng
AU  - Jiao P
AD  - Institute of Atherosclerosis, Taishan Medical University, Taian, China.
FAU - Li, Dong
AU  - Li D
AD  - Institute of Epidemiology, School of Public Health, Taishan Medical University, 
      Taian, China.
FAU - Jiang, Baofa
AU  - Jiang B
AD  - Institute of Epidemiology, School of Public Health, Shandong University, Jinan, 
      China bjiang@sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130322
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (Aurovertins)
RN  - 0 (TNF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - OWX7Q6CF4F (citreoviridin)
SB  - IM
MH  - Atherosclerosis/chemically induced/metabolism
MH  - Aurovertins/*toxicity
MH  - Cell Adhesion/*drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Disease Progression
MH  - Human Umbilical Vein Endothelial Cells/drug effects/*metabolism
MH  - Humans
MH  - Tumor Necrosis Factor-alpha/*drug effects
OTO - NOTNLM
OT  - Citreoviridin
OT  - adhesion
OT  - atherosclerosis
OT  - nuclear factor-kappaB
OT  - vascular endothelial cell
EDAT- 2013/03/26 06:00
MHDA- 2016/01/28 06:00
CRDT- 2013/03/26 06:00
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2016/01/28 06:00 [medline]
AID - 0748233713483194 [pii]
AID - 10.1177/0748233713483194 [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2015 Mar;31(3):193-201. doi: 10.1177/0748233713483194. Epub 
      2013 Mar 22.