PMID- 2352496
OWN - NLM
STAT- MEDLINE
DCOM- 19900716
LR  - 20190820
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 34
IP  - 3
DP  - 1990
TI  - Enhancement of cytotoxicity of active macrophages by mycoplasma: role of 
      mycoplasma-associated induction of tumor necrosis factor-alpha (TNF-alpha) in 
      macrophages.
PG  - 231-43
AB  - Tumor necrosis factor-alpha (TNF-alpha)-inducing activity of several mycoplasmas 
      including Mycoplasma pneumoniae, a causative agent in human respiratory 
      infectious diseases, was investigated. Purified peritoneal macrophages from 
      BALB/c mice markedly enhanced their cytotoxic activity to Meth A cells, when 
      cultured with either viable or non-viable mycoplasmas. The supernatants of the 
      macrophage culture with mycoplasmas, M. pneumoniae and Acholeplasma laidlawii, 
      showed the potent cytotoxic activity to TNF-alpha-sensitive L cells but not to 
      TNF-alpha-insensitive L cells. Addition of anti-TNF-alpha antiserum inhibited 
      completely the cytotoxic activity of these supernatants, indicating that a major 
      part of the cytotoxic activity might be due to TNF-alpha. Various other 
      mycoplasmas, either glucose- or arginine-utilizing species, as far as tested 
      showed also the potent activity to produce TNF-alpha. These results strongly 
      suggest the possibility that mycoplasmas possess the activity of TNF-alpha 
      induction which might be responsible for a part of enhancement of cytotoxic 
      activity of macrophages and resistance to infection with mycoplasmas in vivo.
FAU - Arai, S
AU  - Arai S
AD  - Department of Microbiology, Kurume University, School of Medicine, Fukuoka.
FAU - Furukawa, M
AU  - Furukawa M
FAU - Munakata, T
AU  - Munakata T
FAU - Kuwano, K
AU  - Kuwano K
FAU - Inoue, H
AU  - Inoue H
FAU - Miyazaki, T
AU  - Miyazaki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Antibodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Cells, Cultured
MH  - Colony Count, Microbial
MH  - *Cytotoxicity, Immunologic
MH  - Dose-Response Relationship, Immunologic
MH  - Macrophage Activation
MH  - Macrophages/*immunology/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycoplasma/*physiology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/immunology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1111/j.1348-0421.1990.tb01006.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 1990;34(3):231-43. doi: 10.1111/j.1348-0421.1990.tb01006.x.