PMID- 23525727
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130326
LR  - 20211021
IS  - 0912-0009 (Print)
IS  - 1880-5086 (Electronic)
IS  - 0912-0009 (Linking)
VI  - 52
IP  - 2
DP  - 2013 Mar
TI  - Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation 
      of NF-kappaB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue.
PG  - 112-9
LID - 10.3164/jcbn.12-95 [doi]
AB  - Apurinic/apyrimidinic endonuclease-1 (APE-1), a key enzyme responsible for DNA 
      base excision repair (BER), has been linked to cancer chemoradiosensitivity. The 
      phosphorylation of p65 plays a role in the activation of this pathway. In this 
      study, we investigated APE-1 expression and its interaction with p65 in 
      esophageal squamous cell carcinoma (ESCC) tissue. The expression of APE-1, p65, 
      p65 nuclear localization sequence (p65-NLS), and monocyte chemoattractant 
      protein-1 (MCP-1) was assessed by immunohistochemical analysis in 67 human ESCC 
      tissue samples. Real-time PCR and western blotting were also performed. p65 siRNA 
      was evaluated to determine the role of p65 in the regulation of APE-1 expression. 
      We found nuclear localization of APE-1 in 89.6% (60/67) of ESCC tissue samples. 
      We also observed the colocalization of p65-NLS and APE-1 in esophageal cancer 
      tissue. In KYSE220 cells, pretreatment of MG-132 significantly abrogated 
      upregulation of p65 and APE-1 levels induced by MCP-1, and treatment with 10 and 
      20 nM p65 siRNA significantly inhibited APE-1 mRNA expression. siRNA for p65 
      treatment significantly increased the apoptotic index in 5-FU-treated KYSE220 
      cells. We conclude that APE-1 is overexpressed and mainly localized in the 
      nuclear compartment of cancer cells, and partly regulated by p65 in the NF-kappaB 
      pathway in ESCC tissue.
FAU - Song, Junmin
AU  - Song J
AD  - Division of Gastroenterology, Department of Internal Medicine, Nippon Medical 
      School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan ; Department of 
      Gastroenterology, Shengjing Hospital, China Medical University, Sanhao Street 36, 
      Heping District, Shenyang, 110004, P.R. China.
FAU - Futagami, Seiji
AU  - Futagami S
FAU - Nagoya, Hiroyuki
AU  - Nagoya H
FAU - Kawagoe, Tetsuro
AU  - Kawagoe T
FAU - Yamawaki, Hiroshi
AU  - Yamawaki H
FAU - Kodaka, Yasuhiro
AU  - Kodaka Y
FAU - Tatsuguchi, Atsushi
AU  - Tatsuguchi A
FAU - Gudis, Katya
AU  - Gudis K
FAU - Wakabayashi, Taiga
AU  - Wakabayashi T
FAU - Yonezawa, Masaoki
AU  - Yonezawa M
FAU - Shimpuku, Mayumi
AU  - Shimpuku M
FAU - Watarai, Yasuhiko
AU  - Watarai Y
FAU - Iwakiri, Katsuhiko
AU  - Iwakiri K
FAU - Hoshihara, Yoshio
AU  - Hoshihara Y
FAU - Makino, Hiroshi
AU  - Makino H
FAU - Miyashita, Masao
AU  - Miyashita M
FAU - Tsuchiya, Shinichi
AU  - Tsuchiya S
FAU - Li, Yan
AU  - Li Y
FAU - E Crowe, Sheila
AU  - E Crowe S
FAU - Sakamoto, Choitsu
AU  - Sakamoto C
LA  - eng
PT  - Journal Article
DEP - 20130301
PL  - Japan
TA  - J Clin Biochem Nutr
JT  - Journal of clinical biochemistry and nutrition
JID - 8700907
PMC - PMC3593127
OTO - NOTNLM
OT  - APE-1
OT  - MCP-1
OT  - NF-kappaB-p65
OT  - esophageal squamous cell carcinoma
OT  - p65-NLS
EDAT- 2013/03/26 06:00
MHDA- 2013/03/26 06:01
PMCR- 2013/03/01
CRDT- 2013/03/26 06:00
PHST- 2012/08/31 00:00 [received]
PHST- 2012/12/18 00:00 [accepted]
PHST- 2013/03/26 06:00 [entrez]
PHST- 2013/03/26 06:00 [pubmed]
PHST- 2013/03/26 06:01 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - jcbn12-95 [pii]
AID - 10.3164/jcbn.12-95 [doi]
PST - ppublish
SO  - J Clin Biochem Nutr. 2013 Mar;52(2):112-9. doi: 10.3164/jcbn.12-95. Epub 2013 Mar 
      1.