PMID- 23528210
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20130326
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 22
IP  - 4
DP  - 2013 Apr
TI  - E-FABP induces differentiation in normal human keratinocytes and modulates the 
      differentiation process in psoriatic keratinocytes in vitro.
PG  - 255-61
LID - 10.1111/exd.12111 [doi]
AB  - Epidermal fatty acid-binding protein (E-FABP) is a lipid carrier, originally 
      discovered in human epidermis. We show that E-FABP is almost exclusively 
      expressed in postmitotic (PM) keratinocytes, corresponding to its localization in 
      the highest suprabasal layers, while it is barely expressed in keratinocyte stem 
      cells (KSC) and transit amplifying (TA) keratinocytes. Transfection of normal 
      human keratinocytes with recombinant (r) E-FABP induces overexpression of K10 and 
      involucrin. On the other hand, E-FABP inhibition by siRNA downregulates K10 and 
      involucrin expression in normal keratinocytes through NF-kappaB and JNK signalling 
      pathways. E-FABP is highly expressed in psoriatic epidermis, and it is mainly 
      localized in stratum spinosum. Psoriatic PM keratinocytes overexpress E-FABP as 
      compared to the same population in normal epidermis. E-FABP inhibition in 
      psoriatic keratinocytes markedly reduces differentiation, while it upregulates 
      psoriatic markers such as survivin and K16. However, under high-calcium 
      conditions, E-FABP silencing downregulates K10 and involucrin, while survivin and 
      K16 expression is completely abolished. These data strongly indicate that E-FABP 
      plays an important role in keratinocyte differentiation. Moreover, E-FABP 
      modulates differentiation in psoriatic keratinocytes.
CI  - (c) 2013 John Wiley & Sons A/S.
FAU - Dallaglio, Katiuscia
AU  - Dallaglio K
AD  - Institute of Dermatology, School of Medicine, University of Modena and Reggio 
      Emilia, Modena, Italy.
FAU - Marconi, Alessandra
AU  - Marconi A
FAU - Truzzi, Francesca
AU  - Truzzi F
FAU - Lotti, Roberta
AU  - Lotti R
FAU - Palazzo, Elisabetta
AU  - Palazzo E
FAU - Petrachi, Tiziana
AU  - Petrachi T
FAU - Saltari, Annalisa
AU  - Saltari A
FAU - Coppini, Maurizio
AU  - Coppini M
FAU - Pincelli, Carlo
AU  - Pincelli C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (FABP5 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (KRT10 protein, human)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Recombinant Proteins)
RN  - 147785-83-9 (Keratin-10)
RN  - 60108-77-2 (involucrin)
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Fatty Acid-Binding Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - Humans
MH  - Keratin-10/metabolism
MH  - Keratinocytes/*cytology/pathology/*physiology
MH  - Protein Precursors/metabolism
MH  - Psoriasis/*metabolism/*pathology
MH  - RNA, Small Interfering/genetics
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 2013/03/27 06:00
MHDA- 2013/09/17 06:00
CRDT- 2013/03/27 06:00
PHST- 2013/02/07 00:00 [accepted]
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
AID - 10.1111/exd.12111 [doi]
PST - ppublish
SO  - Exp Dermatol. 2013 Apr;22(4):255-61. doi: 10.1111/exd.12111.