PMID- 23528943
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20151119
IS  - 1544-2217 (Electronic)
IS  - 0300-9858 (Linking)
VI  - 50
IP  - 6
DP  - 2013 Nov
TI  - Immunohistochemical detection of dendritic cell markers in cattle.
PG  - 1099-108
LID - 10.1177/0300985813482951 [doi]
AB  - Dendritic cells (DCs) are "professional" antigen-presenting cells with a critical 
      role in the regulation of innate and adaptive immune responses and thus have been 
      considered of great interest in the study of a variety of infectious diseases. 
      The objective of this investigation was to characterize the in vivo distribution 
      of DCs in bovine tissues by using potential DC markers to establish a basis for 
      the study of DCs in diseased tissues. Markers evaluated included MHCII, CD208, 
      CD1b, CD205, CNA.42, and S100 protein, the latter 2 being expressed by follicular 
      dendritic cells whose origin and role are different from the rest of 
      hematopoietic DCs. Paraffin wax-embedded tissues from 6 healthy Friesian calves 
      were subjected to the avidin-biotin-peroxidase method, and the most appropriate 
      fixatives, dilutions, and antigen retrieval pretreatments were studied for each 
      of the primary antibodies. The most significant results included the localization 
      of CD208-positive cells not only in the T zone of lymphoid organs but also within 
      lymphoid follicles; CD1b-positive cells were mainly found in thymus and 
      interfollicular areas of some lymph nodes; cells stained with anti-CD205 antibody 
      were scarce, and their location was mainly in nonlymphoid tissues; and CNA.42- 
      and S100 protein-positive cells localized in primary lymphoid follicles and light 
      zones of germinal centers, although showing differences in the staining pattern. 
      Furthermore, MHCII was established as one of the most sensitive markers for any 
      DC of hematopoietic origin. These results increase our understanding of DC 
      immunolabeling and will help in future DC studies of both healthy and diseased 
      tissues.
FAU - Romero-Palomo, F
AU  - Romero-Palomo F
AD  - Departamento de Anatomia y Anatomia Patologica Comparadas, Edificio de Sanidad 
      Animal, Campus de Rabanales, 14014, Cordoba, Spain. Email: jcgomez@uco.es.
FAU - Risalde, M A
AU  - Risalde MA
FAU - Molina, V
AU  - Molina V
FAU - Sanchez-Cordon, P J
AU  - Sanchez-Cordon PJ
FAU - Pedrera, M
AU  - Pedrera M
FAU - Gomez-Villamandos, J C
AU  - Gomez-Villamandos JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130325
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (Lysosomal-Associated Membrane Protein 3)
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, CD/metabolism
MH  - Biomarkers/metabolism
MH  - Cattle
MH  - Cattle Diseases/*metabolism/pathology
MH  - Dendritic Cells/*metabolism
MH  - Dendritic Cells, Follicular/metabolism
MH  - Digestive System/metabolism
MH  - Genes, MHC Class II
MH  - Immunohistochemistry/veterinary
MH  - Integumentary System
MH  - Lymphoid Tissue/metabolism
MH  - Lysosomal-Associated Membrane Protein 3/immunology/isolation & purification
MH  - Male
MH  - Respiratory System/metabolism
MH  - S100 Proteins/immunology/metabolism
OTO - NOTNLM
OT  - CD1b
OT  - CD205
OT  - CD208
OT  - MHCII
OT  - bovine
OT  - dendritic cells
OT  - follicular dendritic cells
OT  - immunohistochemistry
EDAT- 2013/03/27 06:00
MHDA- 2015/05/13 06:00
CRDT- 2013/03/27 06:00
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 0300985813482951 [pii]
AID - 10.1177/0300985813482951 [doi]
PST - ppublish
SO  - Vet Pathol. 2013 Nov;50(6):1099-108. doi: 10.1177/0300985813482951. Epub 2013 Mar 
      25.