PMID- 23529354
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20211021
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 29
IP  - 8
DP  - 2013 Aug
TI  - Halotolerant, alkaliphilic urease-producing bacteria from different climate zones 
      and their application for biocementation of sand.
PG  - 1453-60
LID - 10.1007/s11274-013-1309-1 [doi]
AB  - Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based 
      on urease activity of halotolerant and alkaliphilic microorganisms that can be 
      used for the soil bioclogging and biocementation in geotechnical engineering. 
      However, enrichment cultures produced from indigenous soil bacteria cannot be 
      used for large-scale MICP because their urease activity decreased with the rate 
      about 5 % per one generation. To ensure stability of urease activity in 
      biocement, halotolerant and alkaliphilic strains of urease-producing bacteria for 
      soil biocementation were isolated from either sandy soil or high salinity water 
      in different climate zones. The strain Bacillus sp. VUK5, isolated from soil in 
      Ukraine (continental climate), was phylogenetically close in identity (99 % of 
      16S rRNA gene sequence) to the strain of Bacillus sp. VS1 isolated from beach 
      sand in Singapore (tropical rainforest climate), as well as to the strains of 
      Bacillus sp. isolated by other researchers in Ghent, Belgium (maritime temperate 
      climate) and Yogyakarta, Indonesia (tropical rainforest climate). Both strains 
      Bacillus sp. VS1 and VUK5 had maximum specific growth rate of 0.09/h and maximum 
      urease activities of 6.2 and 8.8 mM of hydrolysed urea/min, respectively. The 
      halotolerant and alkaliphilic strain of urease-producing bacteria isolated from 
      water of the saline lake Dead Sea in Jordan was presented by Gram-positive cocci 
      close to the species Staphylococcus succinus. However, the strains of this 
      species could be hemolytic and toxigenic, therefore only representatives of 
      alkaliphilic Bacillus sp. were used for the biocementation studies. Unconfined 
      compressive strengths for dry biocemented sand samples after six batch treatments 
      with strains VS1and VUK5 were 765 and 845 kPa, respectively. The content of 
      precipitated calcium and the strength of dry biocemented sand at permeability 
      equals to 1 % of initial value were 12.4 g Ca/kg of dry sand and 454 kPa, 
      respectively, in case of biocementation by the strain VS1. So, halotolerant, 
      alkaliphilic, urease-producing bacteria isolated from different climate zones 
      have similar properties and can be used for biocementation of soil.
FAU - Stabnikov, Viktor
AU  - Stabnikov V
AD  - Department of Biotechnology and Microbiology, National University of Food 
      Technologies, 68 Vladymyrskaya Str., Kiev 01601, Ukraine. vstabnikov@yandex.ru
FAU - Chu, Jian
AU  - Chu J
FAU - Ivanov, Volodymyr
AU  - Ivanov V
FAU - Li, Yishan
AU  - Li Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130326
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 0 (Alkalies)
RN  - 0 (Bacterial Proteins)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
EIN - World J Microbiol Biotechnol. 2014 Apr;30(4):1433. Jian, Chu [corrected to Chu, 
      Jian]
MH  - Alkalies/metabolism
MH  - Bacillus/classification/enzymology/genetics/*isolation & purification
MH  - Bacterial Proteins/chemistry/genetics/*metabolism
MH  - Cementation
MH  - Climate
MH  - Ecosystem
MH  - Enzyme Stability
MH  - Hydrogen-Ion Concentration
MH  - Jordan
MH  - Kinetics
MH  - Lakes/*microbiology
MH  - Phylogeny
MH  - Silicon Dioxide/*chemistry
MH  - Sodium Chloride/metabolism
MH  - *Soil Microbiology
MH  - Staphylococcus/classification/enzymology/genetics/*isolation & purification
MH  - Urease/chemistry/genetics/*metabolism
EDAT- 2013/03/27 06:00
MHDA- 2014/02/05 06:00
CRDT- 2013/03/27 06:00
PHST- 2013/01/14 00:00 [received]
PHST- 2013/03/07 00:00 [accepted]
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
AID - 10.1007/s11274-013-1309-1 [doi]
PST - ppublish
SO  - World J Microbiol Biotechnol. 2013 Aug;29(8):1453-60. doi: 
      10.1007/s11274-013-1309-1. Epub 2013 Mar 26.