PMID- 23529443
OWN - NLM
STAT- MEDLINE
DCOM- 20130905
LR  - 20220310
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 998
DP  - 2013
TI  - Recording ion channels in isolated, split-opened tubules.
PG  - 341-53
LID - 10.1007/978-1-62703-351-0_27 [doi]
AB  - Ion channels play key roles in physiology. They function as protein transducers 
      able to transform stimuli and chemical gradients into electrical signals. They 
      also are critical for cell signaling and play a particularly important role in 
      epithelial transport acting as gateways for the movement of electrolytes across 
      epithelial cell membranes. Experimental limitations, though, have hampered the 
      recording of ion channel activity in many types of tissue. This has slowed 
      progress in understanding the cellular and physiological function of these 
      channels with most function inferred from in vitro systems and cell culture 
      models. In many cases, such inferences have clouded rather than clarified the 
      picture. Here, we describe a contemporary method for isolating and patch-clamping 
      renal tubules for ex vivo analysis of ion channel function in native tissue. 
      Focus is placed on quantifying the activity of the epithelial Na(+) channel 
      (ENaC) in the aldosterone--sensitive distal nephron (ASDN). This isolated, 
      split-open tubule preparation enables recording of renal ion channels in the 
      close-to-native environment under the control of native cell signaling pathways 
      and receptors. When combined with complementary measurements of organ and system 
      function, and contemporary molecular genetics and pharmacology used to manipulate 
      function and regulation, patch-clamping renal channels in the isolated, 
      split-open tubule enables understanding to emerge about the physiological 
      function of these key proteins from the molecule to the whole animal.
FAU - Mironova, Elena
AU  - Mironova E
AD  - Department of Physiology, University of Texas Health Science Center at San 
      Antonio, San Antonio, TX, USA.
FAU - Bugay, Vladislav
AU  - Bugay V
FAU - Pochynyuk, Oleh
AU  - Pochynyuk O
FAU - Staruschenko, Alexander
AU  - Staruschenko A
FAU - Stockand, James D
AU  - Stockand JD
LA  - eng
GR  - R01 DK087460/DK/NIDDK NIH HHS/United States
GR  - R01 DK059594/DK/NIDDK NIH HHS/United States
GR  - R01 DK070571/DK/NIDDK NIH HHS/United States
GR  - R01 HL108880/HL/NHLBI NIH HHS/United States
GR  - R01 DK59594/DK/NIDDK NIH HHS/United States
GR  - R01HL108880/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Ion Channels)
SB  - IM
MH  - Animals
MH  - Dissection
MH  - Ion Channels/*metabolism
MH  - Kidney Tubules/*cytology/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Patch-Clamp Techniques/*methods
PMC - PMC4181527
MID - NIHMS630072
EDAT- 2013/03/27 06:00
MHDA- 2013/09/06 06:00
PMCR- 2014/10/01
CRDT- 2013/03/27 06:00
PHST- 2013/03/27 06:00 [entrez]
PHST- 2013/03/27 06:00 [pubmed]
PHST- 2013/09/06 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 10.1007/978-1-62703-351-0_27 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;998:341-53. doi: 10.1007/978-1-62703-351-0_27.