PMID- 23531004
OWN - NLM
STAT- MEDLINE
DCOM- 20130517
LR  - 20211021
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1279
DP  - 2013 Mar
TI  - Imaging spinal neuron ensembles active during locomotion with genetically encoded 
      calcium indicators.
PG  - 71-9
LID - 10.1111/nyas.12092 [doi]
AB  - Advances in molecular-genetic tools for labeling neuronal subtypes, and the 
      emerging development of robust genetic probes for neural activity, are likely to 
      revolutionize our understanding of the functional organization of neural 
      circuits. In principle, these tools should be able to detect activity at cellular 
      resolution for large ensembles of identified neuron types as they participate in 
      specific behaviors. This report describes the use of genetically encoded calcium 
      indicators (GECIs), combined with two-photon microscopy, to characterize V1 
      interneurons, known to be critical for setting the duration of the step cycle. 
      All V1 interneurons arise from a common precursor population and express 
      engrailed-1 (En1). Our data show that although neighboring interneurons that 
      arise from the same developmental lineage and share many features, such as 
      projection patterns and neurotransmitter profiles, they are not irrevocably 
      committed to having the same pattern of activity.
CI  - (c) 2013 New York Academy of Sciences.
FAU - Hinckley, Christopher A
AU  - Hinckley CA
AD  - Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk 
      Institute for Biological Studies, La Jolla, CA 92037, USA.
FAU - Pfaff, Samuel L
AU  - Pfaff SL
LA  - eng
GR  - R01 MH099571/MH/NIMH NIH HHS/United States
GR  - R01 NS054172/NS/NINDS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R37 NS037116/NS/NINDS NIH HHS/United States
GR  - P30 CA014195/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Indicators and Reagents)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Calcium Signaling/*genetics
MH  - *Genes, Reporter
MH  - Humans
MH  - Indicators and Reagents/analysis/*metabolism
MH  - *Locomotion/genetics/physiology
MH  - Motor Neurons/cytology/*physiology
MH  - *Optical Imaging/methods
MH  - Spinal Cord/*cytology/physiology
MH  - Voltage-Sensitive Dye Imaging/methods
PMC - PMC3908931
MID - NIHMS446413
EDAT- 2013/03/28 06:00
MHDA- 2013/05/18 06:00
PMCR- 2014/04/01
CRDT- 2013/03/28 06:00
PHST- 2013/03/28 06:00 [entrez]
PHST- 2013/03/28 06:00 [pubmed]
PHST- 2013/05/18 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1111/nyas.12092 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2013 Mar;1279:71-9. doi: 10.1111/nyas.12092.