PMID- 23534256
OWN - NLM
STAT- MEDLINE
DCOM- 20130917
LR  - 20151008
IS  - 0029-0203 (Print)
IS  - 0029-0203 (Linking)
VI  - 117
IP  - 2
DP  - 2013 Feb
TI  - [Evaluation of chemokines in tears of patients with infectious keratitis].
PG  - 117-25
AB  - PURPOSE: To investigate the chemokine profile in tears of patients with 
      infectious keratitis. SUBJECTS AND METHODS: Subjects were 32 eyes of 16 patients 
      with infectious keratitis and 5 eyes of 5 healthy volunteers as a control. The 
      patients with infectious keratitis were classified into two groups of eyes: 10 
      with bacterial keratitis and 6 with Acanthamoeba keratitis. Tear fluid was 
      obtained from both eyes of the patients with infectious keratitis and from the 
      right eyes of the control subjects using filter paper. Chemokine concentration 
      (unit: Odu/mm2) and its profile in tears was analyzed using an antibody-array. 
      RESULTS: In terms of chemokine profile in the bacterial keratitis group, the 
      expression volume of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 
      (MCP-1) in the diseased eyes was significantly higher than in the healthy eyes (p 
      < 0.05). The expression volume of mucosae-associated epithelial chemokines (MECs) 
      in the diseased eyes of the bacterial keratitis group was significantly lower 
      than in the healthy eyes of that group (p < 0.05). In the Acanthamoeba keratitis 
      group, chemokines were not significantly increased in the diseased eyes compared 
      with those in the healthy eyes. However, MCP-1 was increased in tears of the 
      Acanthamoeba keratitis group. Regarding the chemokine ratio, the IL-8/MEC ratio 
      in the diseased eyes of the Pseudomonas keratitis group and the MCP-1/IL-8 in the 
      diseased eyes of the Acanthamoeba keratitis group showed a significantly high 
      level (p < 0.05). CONCLUSION: We concluded that the analyses of the chemokine 
      profile and chemokine ratio in the tears of infectious keratitis patients is 
      useful as a clinical tear laboratory test to interpret the pathologic condition 
      of infectious keratitis
FAU - Hori, Shinsuke
AU  - Hori S
AD  - Division of Ophthalmology, Department of Visual Sciences, Nihon University School 
      of Medicine, Tokyo, Japan. qqfv3dsd@tuba.ocn.ne.jp
FAU - Shoji, Jun
AU  - Shoji J
FAU - Inada, Noriko
AU  - Inada N
FAU - Sawa, Mitsuru
AU  - Sawa M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nippon Ganka Gakkai Zasshi
JT  - Nippon Ganka Gakkai zasshi
JID - 7505716
RN  - 0 (Antibodies)
RN  - 0 (Chemokines)
SB  - IM
MH  - Adult
MH  - Antibodies/metabolism
MH  - Chemokines/*metabolism
MH  - Eye Infections/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Keratitis/*metabolism
MH  - Male
MH  - Tears/*metabolism
EDAT- 2013/03/29 06:00
MHDA- 2013/09/18 06:00
CRDT- 2013/03/29 06:00
PHST- 2013/03/29 06:00 [entrez]
PHST- 2013/03/29 06:00 [pubmed]
PHST- 2013/09/18 06:00 [medline]
PST - ppublish
SO  - Nippon Ganka Gakkai Zasshi. 2013 Feb;117(2):117-25.