PMID- 23536218
OWN - NLM
STAT- MEDLINE
DCOM- 20140127
LR  - 20181203
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 37
IP  - 8
DP  - 2013 Aug
TI  - Experimental study on the facilitative effects of miR-125b on the differentiation 
      of rat bone marrow mesenchymal stem cells into neuron-like cells.
PG  - 812-9
LID - 10.1002/cbin.10103 [doi]
AB  - To observe the effects of miR-125b on the differentiation of rat bone marrow 
      mesenchymal stem cells (BMSCs) into neuron-like cells, rat BMSCs were isolated 
      and transfected with Syn-rno-miR-125b* miScript miRNA Mimic (Mimic + BME group), 
      with anti-rno-miR-125b* miScript miRNA Inhibitor (Inhibitor + BME group) and BME 
      control was set up without transfection. Blank controls without transfection and 
      induction by BME was also set up. BMSCs of three groups including Mimic + BME 
      group, Inhibitor + BME group and BME group were induced to differentiate into 
      neuron-like cells by beta-mercaptoethanol (BME). Cells in the Blank group were not 
      treated by BME. mRNA expression of miR-125b was determined with qRT-PCR in each 
      group. mRNA and protein expressions of seven nerve cell markers, including beta3 
      tubulin, neural microtubule-associated protein (MAP-2), neurofilament protein 
      (NF), neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), 
      Nestin and Vimentin in the four groups were determined by RT-PCR and Western 
      blots. GFAP and Nestin were detected by immunofluorescent and immunocytochemistry 
      assays. Compared with BME group, mRNA and protein expression of beta3 tubulin, 
      MAP-2, NF, NSE, GFAP, Nestin were significantly increased in the Mimic + BME 
      group (P < 0.01), but significantly decreased in the Inhibitor + BME group (P < 
      0.01). Cells in the Mimic + BME group were more like nerve cells in morphology 
      than cells in the BME groups. Thus miR-125b can promote BME to induce rat BMSCs 
      differentiation into neuron-like cells.
CI  - (c) 2013 International Federation for Cell Biology.
FAU - Wu, Rui
AU  - Wu R
AD  - Department of Neurology, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, 450052, P.R., China.
FAU - Wang, Na
AU  - Wang N
FAU - Li, Min
AU  - Li M
FAU - Zang, Wenqiao
AU  - Zang W
FAU - Xu, Yuming
AU  - Xu Y
LA  - eng
PT  - Journal Article
DEP - 20130418
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Antigens, Differentiation)
RN  - 0 (MIRN125 microRNA, rat)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/metabolism
MH  - Bone Marrow Cells/physiology
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression
MH  - Male
MH  - Mesenchymal Stem Cells/*physiology
MH  - MicroRNAs/*genetics/metabolism
MH  - Neurons/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - BMSCs
OT  - differentiation
OT  - miR-125b
EDAT- 2013/03/29 06:00
MHDA- 2014/01/28 06:00
CRDT- 2013/03/29 06:00
PHST- 2012/10/13 00:00 [received]
PHST- 2013/03/09 00:00 [accepted]
PHST- 2013/03/29 06:00 [entrez]
PHST- 2013/03/29 06:00 [pubmed]
PHST- 2014/01/28 06:00 [medline]
AID - 10.1002/cbin.10103 [doi]
PST - ppublish
SO  - Cell Biol Int. 2013 Aug;37(8):812-9. doi: 10.1002/cbin.10103. Epub 2013 Apr 18.