PMID- 23536661
OWN - NLM
STAT- MEDLINE
DCOM- 20130730
LR  - 20240318
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 11
DP  - 2013 Jun
TI  - Identification and characterization of genetically divergent members of the newly 
      established family Mesoniviridae.
PG  - 6346-58
LID - 10.1128/JVI.00416-13 [doi]
AB  - The recently established family Mesoniviridae (order Nidovirales) contains a 
      single species represented by two closely related viruses, Cavally virus (CavV) 
      and Nam Dinh virus (NDiV), which were isolated from mosquitoes collected in Cote 
      d'Ivoire and Vietnam, respectively. They represent the first nidoviruses to be 
      discovered in insects. Here, we report the molecular characterization of four 
      novel mesoniviruses, Hana virus, Meno virus, Nse virus, and Moumo virus, all of 
      which were identified in a geographical region in Cote d'Ivoire with high CavV 
      prevalence. The viruses were found with prevalences between 0.5 and 2.8%, and 
      genome sequence analyses and phylogenetic studies suggest that they represent at 
      least three novel species. Electron microscopy revealed prominent club-shaped 
      surface projections protruding from spherical, enveloped virions of about 120 nm. 
      Northern blot data show that the four mesoniviruses analyzed in this study 
      produce two major 3'-coterminal subgenomic mRNAs containing two types of 5' 
      leader sequences resulting from the use of different pairs of leader and body 
      transcription-regulating sequences that are conserved among mesoniviruses. 
      Protein sequencing, mass spectroscopy, and Western blot data show that 
      mesonivirus particles contain eight major structural protein species, including 
      the putative nucleocapsid protein (25 kDa), differentially glycosylated forms of 
      the putative membrane protein (20, 19, 18, and 17 kDa), and the putative spike 
      (S) protein (77 kDa), which is proteolytically cleaved at a conserved site to 
      produce S protein subunits of 23 and 57 kDa. The data provide fundamental new 
      insight into common and distinguishing biological properties of members of this 
      newly identified virus family.
FAU - Zirkel, Florian
AU  - Zirkel F
AD  - Institute of Virology, University of Bonn Medical Center, Bonn, Germany.
FAU - Roth, Hanna
AU  - Roth H
FAU - Kurth, Andreas
AU  - Kurth A
FAU - Drosten, Christian
AU  - Drosten C
FAU - Ziebuhr, John
AU  - Ziebuhr J
FAU - Junglen, Sandra
AU  - Junglen S
LA  - eng
SI  - GENBANK/JQ957872
SI  - GENBANK/JQ957873
SI  - GENBANK/JQ957874
SI  - GENBANK/KC768950
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130327
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cote d'Ivoire
MH  - Culicidae/*virology
MH  - *Evolution, Molecular
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nidovirales/classification/*genetics/*isolation & purification
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Viral Structural Proteins/genetics
PMC - PMC3648093
EDAT- 2013/03/29 06:00
MHDA- 2013/07/31 06:00
PMCR- 2013/12/01
CRDT- 2013/03/29 06:00
PHST- 2013/03/29 06:00 [entrez]
PHST- 2013/03/29 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - JVI.00416-13 [pii]
AID - 00416-13 [pii]
AID - 10.1128/JVI.00416-13 [doi]
PST - ppublish
SO  - J Virol. 2013 Jun;87(11):6346-58. doi: 10.1128/JVI.00416-13. Epub 2013 Mar 27.