PMID- 23536686
OWN - NLM
STAT- MEDLINE
DCOM- 20130730
LR  - 20220129
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 11
DP  - 2013 Jun
TI  - A comparison of murine leukemia viruses that escape from human and rhesus macaque 
      TRIM5alphas.
PG  - 6455-68
LID - 10.1128/JVI.03425-12 [doi]
AB  - To better understand the binding mechanism of TRIM5alpha to retrovirus capsid, we had 
      previously selected N-tropic murine leukemia virus (N-MLV) mutants escaping from 
      rhesus macaque TRIM5alpha (rhTRIM5alpha) by passaging the virus in rhTRIM5alpha-expressing 
      cells and selecting for nonrestricted variants. To test the commonality of the 
      findings from the rhTRIM5alpha study, we have now employed a similar genetic approach 
      using human TRIM5alpha (huTRIM5alpha). Consistent with the rhTRIM5alpha study, the mapped 
      huTRIM5alpha escape mutations were distributed across the capsid exterior, confirming 
      the extended binding surface between virus and restriction factor. Compared to 
      the results of the previous study, fewer escape mutations were identified, with 
      particular mutants being repeatedly selected. Three out four huTRIM5alpha escape 
      variants showed resistance to all primate TRIM5alphas tested, but two of them 
      sacrificed viral fitness, observations that were not made in the rhTRIM5alpha study. 
      Moreover, differences in amino acid changes associated with escape from hu- and 
      rhTRIM5alphas suggested a charge dependence of the restriction by different TRIM5alphas. 
      Taken together, these results suggest that the recognition of the entire capsid 
      surface is a general strategy for TRIM5alpha to restrict MLV but that significantly 
      different specific interactions are involved in the binding of TRIM5alpha from 
      different species to the MLV capsid core.
FAU - Ohkura, Sadayuki
AU  - Ohkura S
AD  - Division of Virology, MRC National Institute for Medical Research, Mill Hill, 
      London, United Kingdom.
FAU - Stoye, Jonathan P
AU  - Stoye JP
LA  - eng
GR  - MC_U117512710/MRC_/Medical Research Council/United Kingdom
GR  - U117512710/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130327
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Capsid Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - Antiviral Restriction Factors
MH  - Capsid Proteins/genetics/*metabolism
MH  - Carrier Proteins/genetics/metabolism
MH  - Humans
MH  - Leukemia Virus, Murine/genetics/*metabolism
MH  - Macaca mulatta
MH  - Models, Molecular
MH  - Protein Binding
MH  - Retroviridae Infections/genetics/*metabolism/virology
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases
PMC - PMC3648092
EDAT- 2013/03/29 06:00
MHDA- 2013/07/31 06:00
PMCR- 2013/12/01
CRDT- 2013/03/29 06:00
PHST- 2013/03/29 06:00 [entrez]
PHST- 2013/03/29 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - JVI.03425-12 [pii]
AID - 03425-12 [pii]
AID - 10.1128/JVI.03425-12 [doi]
PST - ppublish
SO  - J Virol. 2013 Jun;87(11):6455-68. doi: 10.1128/JVI.03425-12. Epub 2013 Mar 27.