PMID- 23536985
OWN - NLM
STAT- MEDLINE
DCOM- 20140110
LR  - 20130614
IS  - 1556-7125 (Electronic)
IS  - 1535-3141 (Linking)
VI  - 10
IP  - 6
DP  - 2013 Jun
TI  - Role of capsular polysaccharides and lipooligosaccharides in Campylobacter 
      surface properties, autoagglutination, and attachment to abiotic surfaces.
PG  - 506-13
LID - 10.1089/fpd.2012.1365 [doi]
AB  - The role of capsular polysaccharides and lipooligosaccharides in cell surface 
      hydrophobicity, surface charge, autoagglutination (AAG), and attachment to 
      abiotic surfaces of three strains of Campylobacter jejuni and one strain of C. 
      coli were investigated. This was achieved by removal of capsular polysaccharides 
      and truncation of lipooligosaccharides core oligosaccharides by inactivation of 
      the kpsE and waaF genes, respectively. The mutants and the wild-type strains were 
      compared after growth under planktonic (broth) and sessile (agar) conditions. 
      Cells grown as planktonic cultures showed a significantly (p<0.05) higher degree 
      of hydrophobicity and AAG activity but differed from their sessile counterparts 
      with respect to surface charge and attachment counts, depending on the strain. 
      These results suggest that prior mode of growth affects the surface properties 
      and attachment of Campylobacter in a strain-dependent manner. There were no 
      significant (p>0.05) differences between the three C. jejuni strains and their 
      DeltakpsE and DeltawaaF mutants with respect to all traits tested. Inactivation of the 
      kpsE gene significantly (p<0.05) reduced the surface charge of the C. coli strain 
      from  approximately -10 to  approximately -6 mV and increased its AAG activity, while disruption of the waaF 
      gene significantly (p<0.05) increased its surface hydrophobicity by >8 degrees  and 
      decreased the numbers of cells attaching to stainless steel and glass by  approximately 0.5 
      log/cm(2). These results suggest that surface polysaccharides may influence the 
      surface properties and attachment to abiotic surfaces of C. coli but not C. 
      jejuni. This suggestion, however, requires further investigation using a larger 
      number of strains of both species.
FAU - Nguyen, Vu Tuan
AU  - Nguyen VT
AD  - CSIRO Food and Nutritional Sciences, Cooper Plains, Queensland, Australia.
FAU - Barlow, Robert S
AU  - Barlow RS
FAU - Fegan, Narelle
AU  - Fegan N
FAU - Turner, Mark S
AU  - Turner MS
FAU - Dykes, Gary A
AU  - Dykes GA
LA  - eng
PT  - Journal Article
DEP - 20130328
PL  - United States
TA  - Foodborne Pathog Dis
JT  - Foodborne pathogens and disease
JID - 101120121
RN  - 0 (Bacterial Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (lipid-linked oligosaccharides)
RN  - 12597-68-1 (Stainless Steel)
RN  - EC 2.4.- (Glycosyltransferases)
RN  - EC 2.4.99.- (heptosyltransferase)
SB  - IM
MH  - Agglutination
MH  - Bacterial Adhesion
MH  - Bacterial Capsules/*metabolism
MH  - Bacterial Load
MH  - Bacterial Proteins/genetics/metabolism
MH  - Campylobacter coli/chemistry/growth & development/*metabolism
MH  - Campylobacter jejuni/chemistry/growth & development/*metabolism
MH  - *Cooking and Eating Utensils
MH  - Glass/chemistry
MH  - Glycosyltransferases/genetics/metabolism
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Lipopolysaccharides/*metabolism
MH  - Membrane Transport Proteins/genetics/metabolism
MH  - Mutation
MH  - Polysaccharides, Bacterial/*metabolism
MH  - Species Specificity
MH  - Stainless Steel/chemistry
MH  - Surface Properties
MH  - Time Factors
EDAT- 2013/03/30 06:00
MHDA- 2014/01/11 06:00
CRDT- 2013/03/30 06:00
PHST- 2013/03/30 06:00 [entrez]
PHST- 2013/03/30 06:00 [pubmed]
PHST- 2014/01/11 06:00 [medline]
AID - 10.1089/fpd.2012.1365 [doi]
PST - ppublish
SO  - Foodborne Pathog Dis. 2013 Jun;10(6):506-13. doi: 10.1089/fpd.2012.1365. Epub 
      2013 Mar 28.