PMID- 23537127
OWN - NLM
STAT- MEDLINE
DCOM- 20131205
LR  - 20130628
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 9
DP  - 2013 May 7
TI  - Stable isotope-assisted lipidomics combined with nontargeted isotopomer 
      filtering, a tool to unravel the complex dynamics of lipid metabolism.
PG  - 4651-7
LID - 10.1021/ac400293y [doi]
AB  - Investigations of complex metabolic mechanisms and networks have become a focus 
      of research in the postgenomic area, thereby creating an increasing demand for 
      sophisticated analytical approaches. One such tool is lipidomics analysis that 
      provides, a detailed picture of the lipid composition of a system at a given 
      time. Introducing stable isotopes into the studied system can additionally 
      provide information on the synthesis, transformation and degradation of 
      individual lipid species. However, capturing the entire dynamics of lipid 
      networks is still a challenge. We developed and evaluated a novel strategy for 
      the in-depth analysis of the dynamics of lipid metabolism with the capacity for 
      high molecular specificity and network coverage. The general workflow consists of 
      stable isotope-labeling experiments, ultrahigh-performance liquid chromatography 
      (UHPLC)/high-resolution Orbitrap-mass spectrometry (MS) lipid profiling and data 
      processing by a software tool for global isotopomer filtering and matching. As a 
      proof of concept, this approach was applied to the network-wide mapping of 
      dynamic lipid metabolism in primary human skeletal muscle cells cultured for 4, 
      12, and 24 h with [U-(13)C]-palmitate. In the myocellular lipid extracts, 692 
      isotopomers were detected that could be assigned to 203 labeled lipid species 
      spanning 12 lipid (sub)classes. Interestingly, some lipid classes showed high 
      turnover rates but stable total amounts while the amount of others increased in 
      the course of palmitate treatment. The novel strategy presented here has the 
      potential to open new detailed insights into the dynamics of lipid metabolism 
      that may lead to a better understanding of physiological mechanisms and metabolic 
      perturbations.
FAU - Li, Jia
AU  - Li J
AD  - CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
FAU - Hoene, Miriam
AU  - Hoene M
FAU - Zhao, Xinjie
AU  - Zhao X
FAU - Chen, Shili
AU  - Chen S
FAU - Wei, Hai
AU  - Wei H
FAU - Haring, Hans-Ulrich
AU  - Haring HU
FAU - Lin, Xiaohui
AU  - Lin X
FAU - Zeng, Zhongda
AU  - Zeng Z
FAU - Weigert, Cora
AU  - Weigert C
FAU - Lehmann, Rainer
AU  - Lehmann R
FAU - Xu, Guowang
AU  - Xu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130412
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Lipids)
SB  - IM
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - *Isotope Labeling
MH  - Lipid Metabolism
MH  - Lipids/*analysis
MH  - Mass Spectrometry
MH  - Muscle, Skeletal/cytology/*metabolism
MH  - Software
MH  - *Thermodynamics
EDAT- 2013/03/30 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/03/30 06:00
PHST- 2013/03/30 06:00 [entrez]
PHST- 2013/03/30 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1021/ac400293y [doi]
PST - ppublish
SO  - Anal Chem. 2013 May 7;85(9):4651-7. doi: 10.1021/ac400293y. Epub 2013 Apr 12.