PMID- 23538322 OWN - NLM STAT- MEDLINE DCOM- 20131212 LR - 20130513 IS - 1872-7077 (Electronic) IS - 1382-6689 (Linking) VI - 36 IP - 1 DP - 2013 Jul TI - Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone. PG - 1-8 LID - S1382-6689(13)00049-5 [pii] LID - 10.1016/j.etap.2013.02.019 [doi] AB - The indiscriminate use of herbicides has led to the contamination of water bodies, possibly affecting the health of aquatic biota. Therefore, to evaluate the possible effects of the clomazone-based herbicide (Gamit((R)) 500) on the fish Prochilodus lineatus, juveniles were exposed for 96h to three concentrations (1, 5 and 10mgL(-1)) of clomazone, and an analysis was made of their hematological parameters: hemoglobin (Hb); hematocrit (Hct); red blood cell (RBC) count; mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC) and biochemical parameters: glutathione S-transferase (GST); catalase (CAT); glutathione peroxidase (GPx) and acetylcholinesterase (AChE). Hct presented a significant decrease at the concentration of 10mgL(-1), while the parameters Hb, HCM and MCHC presented a significant decrease at the two higher concentrations, indicating an anemic condition. The RBC increased significantly at the lowest concentration, possibly due to the release of new red blood cells into the bloodstream in response to splenic contraction, which may occur as an adaptive response to the stressor agent. P. lineatus presented activation of the biotransformation pathway, indicated by augmented hepatic activity of the enzyme GST and hepatic activation of the antioxidant enzyme CAT at the higher concentrations. Liver GPx was significantly inhibited at the higher concentrations, which may indicate the efficient action of CAT in the elimination of H2O2 or its competition with GST for the same substrate (GSH). AChE activity in brain and muscle was inhibited at the higher concentrations, indicating the neurotoxic effects of the herbicide in the fish. The hematological and biochemical alterations led to the conclusion that the herbicide clomazone has toxic effects on the species P. lineatus, and that its presence in the environment may jeopardize the health of these animals. CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Pereira, Lindalva AU - Pereira L AD - Postgraduate Program in Ecology and Natural Resources, Federal University of Sao Carlos (UFSCar), Rodovia Washington Luis, Km 235, CEP: 13565-905, Sao Carlos, SP, Brazil. FAU - Fernandes, Marisa N AU - Fernandes MN FAU - Martinez, Claudia B R AU - Martinez CB LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130307 PL - Netherlands TA - Environ Toxicol Pharmacol JT - Environmental toxicology and pharmacology JID - 9612020 RN - 0 (Hemoglobins) RN - 0 (Herbicides) RN - 0 (Isoxazoles) RN - 0 (Oxazolidinones) RN - 570RAC03NF (clomazone) RN - EC 1.11.1.6 (Catalase) RN - EC 1.11.1.9 (Glutathione Peroxidase) RN - EC 2.5.1.18 (Glutathione Transferase) RN - EC 3.1.1.7 (Acetylcholinesterase) SB - IM MH - Acetylcholinesterase/metabolism MH - Animals MH - Brain/drug effects/enzymology MH - Catalase/metabolism MH - Characiformes/*metabolism MH - Erythrocyte Indices/drug effects MH - Glutathione Peroxidase/metabolism MH - Glutathione Transferase/metabolism MH - Hematocrit MH - Hemoglobins/analysis MH - Herbicides/*toxicity MH - Isoxazoles/*toxicity MH - Liver/drug effects/enzymology MH - Oxazolidinones/*toxicity EDAT- 2013/03/30 06:00 MHDA- 2013/12/18 06:00 CRDT- 2013/03/30 06:00 PHST- 2012/06/18 00:00 [received] PHST- 2013/01/30 00:00 [revised] PHST- 2013/02/28 00:00 [accepted] PHST- 2013/03/30 06:00 [entrez] PHST- 2013/03/30 06:00 [pubmed] PHST- 2013/12/18 06:00 [medline] AID - S1382-6689(13)00049-5 [pii] AID - 10.1016/j.etap.2013.02.019 [doi] PST - ppublish SO - Environ Toxicol Pharmacol. 2013 Jul;36(1):1-8. doi: 10.1016/j.etap.2013.02.019. Epub 2013 Mar 7.