PMID- 23541201
OWN - NLM
STAT- MEDLINE
DCOM- 20130912
LR  - 20191210
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 34
IP  - 2
DP  - 2013 Jun
TI  - Genotyping of dairy Bacillus licheniformis isolates by high resolution melt 
      analysis of multiple variable number tandem repeat loci.
PG  - 344-51
LID - S0740-0020(13)00007-5 [pii]
LID - 10.1016/j.fm.2013.01.006 [doi]
AB  - In dairy foods, the sporeformer Bacillus licheniformis can be the cause of 
      spoilage or specification compliance issues. Currently used methods for 
      genotyping B. licheniformis have limited discrimination with only 2 or 3 
      different subgroups being identified. Here, we have developed a multi-locus 
      variable number tandem repeat analysis (MLVA) method and combined it with high 
      resolution melt analysis (MLV-HRMA) for genotyping B. licheniformis. Five 
      repetitive loci were identified and used as markers for genotyping 52 isolates 
      from two milk powder processing plants and retail samples. Nineteen genotypes 
      could be identified using both MLVA and MLV-HRMA leading to Hunter-Gaston 
      discrimination indices (D-value) of 0.93 each. It was found that all 5 MLVA loci 
      were stable following 10 days of sub-culturing of 8 representative isolates. All 
      isolates were also genotyped using previously used methods including randomly 
      amplified polymorphic DNA-PCR (RAPD) and partial rpoB sequencing. Five different 
      RAPD profiles and 5 different partial rpoB sequence types were identified 
      resulting in corresponding D-values of 0.6 and 0.46, respectively. Analysis of 
      the genotypes from dairy samples revealed that dairy B. licheniformis isolates 
      are more heterogeneous than previously thought and that this new method can 
      potentially allow for more discriminatory tracking and monitoring of specific 
      genotypes.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Dhakal, Rajat
AU  - Dhakal R
AD  - School of Agriculture and Food Sciences, Hartley Teakle Building, Australia. 
      r.dhakal@uq.edu.au
FAU - Chauhan, Kanika
AU  - Chauhan K
FAU - Seale, R Brent
AU  - Seale RB
FAU - Deeth, Hilton C
AU  - Deeth HC
FAU - Pillidge, Christopher J
AU  - Pillidge CJ
FAU - Powell, Ian B
AU  - Powell IB
FAU - Craven, Heather
AU  - Craven H
FAU - Turner, Mark S
AU  - Turner MS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130126
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Animals
MH  - Bacillus/classification/genetics/*isolation & purification
MH  - Bacterial Proteins/genetics
MH  - Bacterial Typing Techniques/*methods
MH  - Cattle
MH  - Food Contamination
MH  - Genotype
MH  - Milk/*microbiology
MH  - *Minisatellite Repeats
MH  - Multilocus Sequence Typing/*methods
MH  - Phylogeny
EDAT- 2013/04/02 06:00
MHDA- 2013/09/13 06:00
CRDT- 2013/04/02 06:00
PHST- 2012/06/07 00:00 [received]
PHST- 2013/01/08 00:00 [revised]
PHST- 2013/01/10 00:00 [accepted]
PHST- 2013/04/02 06:00 [entrez]
PHST- 2013/04/02 06:00 [pubmed]
PHST- 2013/09/13 06:00 [medline]
AID - S0740-0020(13)00007-5 [pii]
AID - 10.1016/j.fm.2013.01.006 [doi]
PST - ppublish
SO  - Food Microbiol. 2013 Jun;34(2):344-51. doi: 10.1016/j.fm.2013.01.006. Epub 2013 
      Jan 26.