PMID- 23542143
OWN - NLM
STAT- MEDLINE
DCOM- 20131023
LR  - 20131121
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 147
IP  - 2
DP  - 2013 May 20
TI  - The incorporation of Brazilian propolis into collagen-based dressing films 
      improves dermal burn healing.
PG  - 419-25
LID - S0378-8741(13)00187-6 [pii]
LID - 10.1016/j.jep.2013.03.031 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Hydroalcoholic solutions of propolis, a resinous 
      product produced by bees, have been currently employed in improving the 
      cicatricial repair. Biological activity of propolis might be related to its 
      antimicrobial, anti-inflammatory and immunomudalatory properties. AIM OF THIS 
      STUDY: Investigate the suitability of the collagen-based films containing 
      hydroalcoholic extracts of two different varieties of Brazilian propolis (green 
      and red ones) on the dermal burn healing in rodent model. MATERIALS AND METHODS: 
      The hydroalcoholic extracts of red propolis (RP) or Green propolis (GP) were 
      incorporated into collagen-based dressing films (COL). Burn wounds were performed 
      in the dorsum of Wistar rats and dressing with COL, COL+GPa (0.5%), COL+GPb 
      (1,0%) or COL+RP (0.5%). A control group (CTR) was performed keeping the wound 
      undressed. The histological analyses were carried out after 3, 7, 14, 21 and 30 
      days for histological assessment of the inflammatory response, epithelization 
      rates (ER), myofibroblastic count (MC) and collagenization pattern. RESULTS: GPa, 
      GPb and RP provided significant decrease of the inflammatory severity, improved 
      the ER in GPa in 7 (p=0.000), 14 (p=0.000), 21 (p=0.005) and 30 days (p=0.015), 
      and induced earlier replacement of type-III for type-I collagen (p<0.05) than COL 
      and CTR. In all the groups, the MC increased progressively from 3 to 14 days, and 
      then started to decrease slowly until 21 days. Although no significant difference 
      was observed among the groups in 3, 7 and 30 days, the MC was significantly 
      increased in RP in 14 (p=0.0001) and 21 days (p=0.04), as well as grosser 
      interlacement of the collagen bundles compared with the other groups. CONCLUSION: 
      The incorporation of hydroalcoholic extracts of Brazilian propolis improved the 
      biological events associated to burn healing without toxic effects, but the red 
      variety provided the best results. Therefore, these collagen-based containing 
      natural apicultural products films may be considered a promising new dressing for 
      wound occlusion and tissue repairing.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - de Almeida, Enrik Barbosa
AU  - de Almeida EB
AD  - University Tiradentes, Aracaju/SE, Brazil. enrik_almeida@yahoo.com.br
FAU - Cordeiro Cardoso, Juliana
AU  - Cordeiro Cardoso J
FAU - Karla de Lima, Adriana
AU  - Karla de Lima A
FAU - de Oliveira, Nivia Lucas
AU  - de Oliveira NL
FAU - de Pontes-Filho, Nicodemos Teles
AU  - de Pontes-Filho NT
FAU - Oliveira Lima, Sonia
AU  - Oliveira Lima S
FAU - Leal Souza, Isana Carla
AU  - Leal Souza IC
FAU - de Albuquerque-Junior, Ricardo Luiz Cavalcanti
AU  - de Albuquerque-Junior RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130327
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Biological Products)
RN  - 0 (Flavonoids)
RN  - 9007-34-5 (Collagen)
RN  - 9009-62-5 (Propolis)
SB  - IM
MH  - Animals
MH  - Bandages
MH  - Biological Products/*administration & dosage
MH  - Brazil
MH  - Burns/*drug therapy
MH  - Cell Differentiation/drug effects
MH  - Collagen/*administration & dosage
MH  - Flavonoids/analysis
MH  - Male
MH  - Myofibroblasts/cytology/drug effects
MH  - Propolis/*chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Skin/injuries
MH  - Wound Healing/*drug effects
EDAT- 2013/04/02 06:00
MHDA- 2013/10/24 06:00
CRDT- 2013/04/02 06:00
PHST- 2012/09/22 00:00 [received]
PHST- 2012/11/14 00:00 [revised]
PHST- 2013/03/10 00:00 [accepted]
PHST- 2013/04/02 06:00 [entrez]
PHST- 2013/04/02 06:00 [pubmed]
PHST- 2013/10/24 06:00 [medline]
AID - S0378-8741(13)00187-6 [pii]
AID - 10.1016/j.jep.2013.03.031 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 May 20;147(2):419-25. doi: 10.1016/j.jep.2013.03.031. Epub 
      2013 Mar 27.