PMID- 23542429
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20130527
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 44
DP  - 2013 Jun
TI  - Characterization of novel 3'untranslated regions and related polymorphisms of the 
      gene NPPC, encoding for the C-type natriuretic peptide.
PG  - 93-9
LID - S0196-9781(13)00087-9 [pii]
LID - 10.1016/j.peptides.2013.03.012 [doi]
AB  - Elevated plasmatic levels of C-type natriuretic peptide (CNP) were found in 
      patients with chronic heart failure (CHF), but its use as sensitive and specific 
      clinical bio-marker is still controversial. In fact, high levels of CNP were also 
      observed in patients classified in low severity New York Heart Association (NYHA) 
      classes. CNP is encoded by a gene poorly studied (NPPC, natriuretic-precursor 
      peptide C), where the regulatory regions are not well defined and the role of 
      single nucleotide polymorphisms (SNPs) poorly ascertained. In the present work, 
      we focused on the characterization of the 3'untranslated region (3'UTR) of the 
      gene, using Rapid Amplification of cDNA 3'-End (3' RACE), and we identified two 
      novel transcript isoforms (L-3'UTR; S-3'UTR; accession number JF420840, HQ419060 
      respectively). Since it could be hypothesized that genetic variations could 
      explain the observed inter-patients differences, we searched for novel SNPs, by 
      the use of High Resolution Melting (HRM). The results showed a complete lack of 
      genetic variations among our series of samples. Moreover, a preliminary 
      evaluation, using literature information and bioinformatic prediction allowed us 
      to predicted the putative relevant microRNAs binding to the novel 3'UTRs that 
      could modulate the post-transcriptional regulation of NPPC and affect the 
      plasmatic levels of CNP. We obtained 750 and 1024 predicted miRNAs targeting the 
      S- and L-3'UTRs, respectively.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Melaiu, O
AU  - Melaiu O
AD  - Department of Biology, University of Pisa, 56126, Italy.
FAU - Facioni, M S
AU  - Facioni MS
FAU - Cabiati, M
AU  - Cabiati M
FAU - Caruso, R
AU  - Caruso R
FAU - Giannessi, D
AU  - Giannessi D
FAU - Landi, S
AU  - Landi S
FAU - Gemignani, F
AU  - Gemignani F
FAU - Del Ry, S
AU  - Del Ry S
LA  - eng
PT  - Journal Article
DEP - 20130328
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MicroRNAs)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA Splice Sites)
RN  - 127869-51-6 (Natriuretic Peptide, C-Type)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Base Sequence
MH  - Binding Sites
MH  - Case-Control Studies
MH  - Cell Line, Tumor
MH  - Conserved Sequence
MH  - Heart Failure/metabolism
MH  - Humans
MH  - MicroRNAs/genetics
MH  - Middle Aged
MH  - Molecular Sequence Annotation
MH  - Molecular Sequence Data
MH  - Myocardium/metabolism
MH  - Natriuretic Peptide, C-Type/*genetics
MH  - *Polymorphism, Single Nucleotide
MH  - Protein Isoforms/genetics
MH  - RNA Interference
MH  - RNA Splice Sites
MH  - Sequence Analysis, DNA
EDAT- 2013/04/02 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/04/02 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2013/03/04 00:00 [revised]
PHST- 2013/03/04 00:00 [accepted]
PHST- 2013/04/02 06:00 [entrez]
PHST- 2013/04/02 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - S0196-9781(13)00087-9 [pii]
AID - 10.1016/j.peptides.2013.03.012 [doi]
PST - ppublish
SO  - Peptides. 2013 Jun;44:93-9. doi: 10.1016/j.peptides.2013.03.012. Epub 2013 Mar 
      28.