PMID- 23545214
OWN - NLM
STAT- MEDLINE
DCOM- 20140129
LR  - 20220321
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 62
IP  - 2
DP  - 2013 May
TI  - Evidence of neurotrophic events due to peritoneal endometriotic lesions.
PG  - 253-61
LID - S1043-4666(13)00105-1 [pii]
LID - 10.1016/j.cyto.2013.03.003 [doi]
AB  - To investigate the neurotrophic properties of endometriosis, as well as the 
      involvement of neurotrophic factors in the development of chronic pelvic pain in 
      patients with endometriosis, we performed a prospective clinical study. The 
      presence of neurotrophins was investigated in the peritoneal fluid (PF) of 
      patients with peritoneal endometriotic lesions or adenomyosis, as well as from 
      women with non-endometriotic adhesions and from women without 
      endometriosis/adenomyosis/adhesions. The PF from patients with peritoneal 
      endometriotic lesions was divided in three groups: asymptomatic endometriosis, 
      minimal pain and severe pain. PF from patients with adenomyosis or with 
      non-endometriotic adhesions and the control group were divided in patients 
      without pain and with pain. Neurotrophin expression in PF was analyzed using 
      Elisa and the neuronal growth assay with cultured chicken sensory ganglia 
      (dorsal-root-ganglia, DRG) and sympathetic ganglia. PF from women with peritoneal 
      endometriotic lesions overexpress nerve growth factor (NGF) and neurotrophin-3 
      (NT-3), but not brain derived neurotrophic factor (BDNF), whereas the PF of women 
      with adenomyosis or adhesions seems to express normal amounts of these factors. 
      Neurotrophin expression did not differ among the pain groups. Furthermore, the PF 
      from patients with peritoneal endometriotic lesions induced a strong sensory and 
      a marginal sympathetic neurite outgrowth, while the PF from women with 
      adenomyosis and non-endometriotic adhesions induced an outgrowth similar to the 
      control group. The induced neurite outgrowth could only be inhibited in DRG 
      incubated with peritoneal endometriotic lesions. Interestingly, the outgrowth of 
      sympathetic ganglia was inhibited in all studied groups. The present study 
      suggests that only peritoneal endometriotic lesions lead to an increased release 
      of NGF and NT-3 into the PF and that NGF modulates the nerve fiber growth in 
      endometriosis.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Barcena de Arellano, Maria Luisa
AU  - Barcena de Arellano ML
AD  - Endometriosis Research Centre Charite, Department of Gynaecology, Charite, Campus 
      Benjamin Franklin, Berlin, Germany. maria-luisa.barcena-de-arellano@charite.de
FAU - Arnold, Julia
AU  - Arnold J
FAU - Lang, Helene
AU  - Lang H
FAU - Vercellino, Giuseppe Filiberto
AU  - Vercellino GF
FAU - Chiantera, Vito
AU  - Chiantera V
FAU - Schneider, Achim
AU  - Schneider A
FAU - Mechsner, Sylvia
AU  - Mechsner S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130330
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adenomyosis/*metabolism
MH  - Adult
MH  - Animals
MH  - Ascitic Fluid/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line
MH  - Chickens
MH  - Chronic Pain
MH  - Endometriosis/*metabolism
MH  - Female
MH  - Ganglia, Spinal/metabolism
MH  - Humans
MH  - Middle Aged
MH  - Neurites/metabolism
MH  - Neurotrophin 3/*metabolism
MH  - Peritoneum/metabolism
MH  - Prospective Studies
MH  - Tissue Adhesions/*metabolism
MH  - Young Adult
EDAT- 2013/04/03 06:00
MHDA- 2014/01/30 06:00
CRDT- 2013/04/03 06:00
PHST- 2012/06/30 00:00 [received]
PHST- 2013/02/25 00:00 [revised]
PHST- 2013/03/03 00:00 [accepted]
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2014/01/30 06:00 [medline]
AID - S1043-4666(13)00105-1 [pii]
AID - 10.1016/j.cyto.2013.03.003 [doi]
PST - ppublish
SO  - Cytokine. 2013 May;62(2):253-61. doi: 10.1016/j.cyto.2013.03.003. Epub 2013 Mar 
      30.