PMID- 23545285
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20181202
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 40
IP  - 3-4
DP  - 2013 Jul-Aug
TI  - Macrophage migration inhibitory factor of Sciaenops ocellatus regulates immune 
      cell trafficking and is involved in pathogen-induced immune response.
PG  - 232-9
LID - S0145-305X(13)00083-9 [pii]
LID - 10.1016/j.dci.2013.03.016 [doi]
AB  - Macrophage migration inhibitory factor (MIF) is a multi-functional cytokine 
      involved in immunoregulation and inflammation. In this study, we examined the 
      expression and biological function of a MIF, SoMIF, from red drum Sciaenops 
      ocellatus. SoMIF is composed of 115 residues and shares 85-99% overall sequence 
      identities with the MIF of a number of teleost. SoMIF expression was detected in 
      a wide range of tissues and upregulated by bacterial and viral infection in a 
      time-dependent manner. In head kidney (HK) leukocytes, pathogen infection induced 
      SoMIF expression, and the expressed SoMIF was secreted into the extracellular 
      milieu. Recombinant SoMIF (rSoMIF) purified from Escherichia coli inhibited the 
      migration of both HK monocytes and lymphocytes, and this inhibitory effect was 
      abolished by the presence of anti-rSoMIF antibodies. When rSoMIF was administered 
      into red drum, it stimulated the production of reactive oxygen species in HK 
      monocytes both in the presence and absence of pathogen infection. In vivo 
      infection study showed that compared to untreated fish, fish pre-treated with 
      rSoMIF before bacterial infection exhibited significantly lower bacterial loads 
      in blood, kidney, spleen, and liver. Taken together, these results indicate that 
      SoMIF is a secreted protein that regulates immune cell trafficking and is 
      involved in pathogen-induced immune response.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Qiu, Reng
AU  - Qiu R
AD  - Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; 
      Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China.
FAU - Li, Jun
AU  - Li J
FAU - Xiao, Zhi-Zhong
AU  - Xiao ZZ
FAU - Sun, Li
AU  - Sun L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130330
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Fish Proteins)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Load
MH  - Bass/*immunology/metabolism
MH  - Cell Movement
MH  - DNA Virus Infections/immunology/*veterinary
MH  - Edwardsiella tarda/immunology
MH  - Enterobacteriaceae Infections/immunology/*veterinary
MH  - Fish Diseases/*immunology/virology
MH  - Fish Proteins/chemistry/*physiology
MH  - Gene Expression
MH  - Gene Expression Regulation/immunology
MH  - Head Kidney/immunology/microbiology
MH  - Immunity, Cellular
MH  - Iridoviridae/immunology
MH  - Leukocytes/immunology/metabolism
MH  - Macrophage Migration-Inhibitory Factors/chemistry/*physiology
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Sequence Homology, Amino Acid
EDAT- 2013/04/03 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/04/03 06:00
PHST- 2012/12/14 00:00 [received]
PHST- 2013/03/18 00:00 [revised]
PHST- 2013/03/22 00:00 [accepted]
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
AID - S0145-305X(13)00083-9 [pii]
AID - 10.1016/j.dci.2013.03.016 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2013 Jul-Aug;40(3-4):232-9. doi: 10.1016/j.dci.2013.03.016. 
      Epub 2013 Mar 30.