PMID- 23545328
OWN - NLM
STAT- MEDLINE
DCOM- 20130725
LR  - 20211021
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 378
IP  - 2
DP  - 2013 Jun 15
TI  - Following the 'tracks': Tramtrack69 regulates epithelial tube expansion in the 
      Drosophila ovary through Paxillin, Dynamin, and the homeobox protein Mirror.
PG  - 154-69
LID - S0012-1606(13)00146-2 [pii]
LID - 10.1016/j.ydbio.2013.03.017 [doi]
AB  - Epithelial tubes are the infrastructure for organs and tissues, and tube 
      morphogenesis requires precise orchestration of cell signaling, shape, migration, 
      and adhesion. Follicle cells in the Drosophila ovary form a pair of epithelial 
      tubes whose lumens act as molds for the eggshell respiratory filaments, or dorsal 
      appendages (DAs). DA formation is a robust and accessible model for studying the 
      patterning, formation, and expansion of epithelial tubes. Tramtrack69 (TTK69), a 
      transcription factor that exhibits a variable embryonic DNA-binding preference, 
      controls DA lumen volume and shape by promoting tube expansion; the tramtrack 
      mutation twin peaks (ttk(twk)) reduces TTK69 levels late in oogenesis, inhibiting 
      this expansion. Microarray analysis of wild-type and ttk(twk) ovaries, followed 
      by in situ hybridization and RNAi of candidate genes, identified the 
      Phospholipase B-like protein Lamina ancestor (LAMA), the scaffold protein 
      Paxillin, the endocytotic regulator Shibire (Dynamin), and the homeodomain 
      transcription factor Mirror, as TTK69 effectors of DA-tube expansion. These genes 
      displayed enriched expression in DA-tube cells, except lama, which was expressed 
      in all follicle cells. All four genes showed reduced expression in ttk(twk) 
      mutants and exhibited RNAi phenotypes that were enhanced in a ttk(twk)/+ 
      background, indicating ttk(twk) genetic interactions. Although previous studies 
      show that Mirror patterns the follicular epithelium prior to DA tubulogenesis, we 
      show that Mirror has an independent, novel role in tube expansion, involving 
      positive regulation of Paxillin. Thus, characterization of 
      ttk(twk)-differentially expressed genes expands the network of TTK69 effectors, 
      identifies novel epithelial tube-expansion regulators, and significantly advances 
      our understanding of this vital developmental process.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Peters, Nathaniel C
AU  - Peters NC
AD  - University of Washington, Molecular and Cellular Biology Program and Department 
      of Genome Sciences, Box 355065, Seattle, WA 98195-5065, USA.
FAU - Thayer, Nathaniel H
AU  - Thayer NH
FAU - Kerr, Scott A
AU  - Kerr SA
FAU - Tompa, Martin
AU  - Tompa M
FAU - Berg, Celeste A
AU  - Berg CA
LA  - eng
GR  - R01 GM079433/GM/NIGMS NIH HHS/United States
GR  - R01-GM079433/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130330
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Pax protein, Drosophila)
RN  - 0 (Paxillin)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (lama protein, Drosophila)
RN  - 0 (mirr protein, Drosophila)
RN  - 0 (ttk protein, Drosophila)
RN  - EC 3.6.5.5 (Dynamins)
RN  - EC 3.6.5.5 (shi protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Drosophila Proteins/*genetics/metabolism
MH  - Drosophila melanogaster/embryology/*genetics/metabolism
MH  - Dynamins/genetics/metabolism
MH  - Epithelium/embryology/*metabolism
MH  - Eye Proteins/genetics/metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - Gene Regulatory Networks
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Models, Genetic
MH  - Mutation
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Ovary/embryology/*metabolism
MH  - Paxillin/genetics/metabolism
MH  - Protein Binding
MH  - RNA Interference
MH  - Repressor Proteins/genetics/metabolism
MH  - Transcription Factors/genetics/metabolism
PMC - PMC4141043
MID - NIHMS478800
EDAT- 2013/04/03 06:00
MHDA- 2013/07/26 06:00
PMCR- 2014/08/21
CRDT- 2013/04/03 06:00
PHST- 2012/11/27 00:00 [received]
PHST- 2013/03/05 00:00 [revised]
PHST- 2013/03/16 00:00 [accepted]
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2013/07/26 06:00 [medline]
PHST- 2014/08/21 00:00 [pmc-release]
AID - S0012-1606(13)00146-2 [pii]
AID - 10.1016/j.ydbio.2013.03.017 [doi]
PST - ppublish
SO  - Dev Biol. 2013 Jun 15;378(2):154-69. doi: 10.1016/j.ydbio.2013.03.017. Epub 2013 
      Mar 30.