PMID- 23545843
OWN - NLM
STAT- MEDLINE
DCOM- 20131105
LR  - 20181217
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 7
IP  - 5
DP  - 2013 May
TI  - Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine 
      (SIN-1)-induced oxidative stress in HIT-T15 pancreatic beta cells.
PG  - 1607-12
LID - 10.3892/mmr.2013.1396 [doi]
AB  - Reactive oxygen species (ROS)-induced pancreatic beta cell death affects insulin 
      secretion and is important in the pathogenesis of diabetes. Lagerstroemia 
      speciosa, a traditional folk medicine, has been used for t he prevention and 
      treatment of diabetes. However, whether Lagerstroemia speciosa has a 
      cytoprotective effect on pancreatic beta cells remains to be elucidated. The present 
      study aimed to investigate the cytoprotective effects of hot water extracts from 
      Lagerstroemia speciosa leaves (LWE) on 3-morpholinosydnonimine (SIN-1)-induced 
      oxidative damage in Syrian hamster pancreatic insulinoma HIT-T15 cells. The 
      HIT-T15 cells were first treated with SIN-1 (50 microM) for 24 h and then 
      co-incubated with LWE for 48 h. SIN-1 significantly decreased HIT-T15 cell 
      viability (P<0.05); however, LWE did not exert a significant cytotoxic effect and 
      increased the viability of HIT-T15 cells in a dose‑dependent manner. To further 
      investigate the protective effects of LWE on SIN-1‑induced oxidative stress in 
      HIT-T15 cells, the cellular levels of ROS, lipid peroxidation and endogenous 
      antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and 
      glutathione peroxidase (GSH-px), were determined. LWE decreased the intracellular 
      levels of ROS and lipid peroxidation, and increased the activities of antioxidant 
      enzymes. These results suggest that LWE has a cytoprotective effect against 
      SIN-1‑induced oxidative stress in HIT-T15 cells through the inhibition of lipid 
      peroxidation, a decrease in ROS levels and an increase in antioxidant enzyme 
      activity. In addition, LWE increased insulin secretion in SIN-1-treated HIT-T15 
      cells. Our results suggested that LWE were effective in the treatment of 
      diabetes. Further studies are required to study the anti-diabetic molecular 
      mechanism in a cell model.
FAU - Song, Jia-Le
AU  - Song JL
AD  - Department of Food Science and Nutrition, Pusan National University, Busan 
      609-735, Republic of Korea.
FAU - Zhao, Xin
AU  - Zhao X
FAU - Wang, Qiang
AU  - Wang Q
FAU - Zhang, Ting
AU  - Zhang T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130326
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antioxidants)
RN  - 0 (Insulin)
RN  - 0 (Plant Extracts)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 5O5U71P6VQ (linsidomine)
RN  - D46583G77X (Molsidomine)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Cell Survival/drug effects
MH  - Cricetinae
MH  - Insulin/metabolism
MH  - Insulin Secretion
MH  - Insulin-Secreting Cells/*drug effects/metabolism/*pathology
MH  - Intracellular Space/drug effects/metabolism
MH  - Lagerstroemia/*chemistry
MH  - Lipid Peroxidation/drug effects
MH  - Malondialdehyde/metabolism
MH  - Mesocricetus
MH  - Molsidomine/analogs & derivatives
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Protective Agents/*pharmacology
MH  - Reactive Oxygen Species/metabolism
EDAT- 2013/04/03 06:00
MHDA- 2013/11/06 06:00
CRDT- 2013/04/03 06:00
PHST- 2012/11/15 00:00 [received]
PHST- 2013/03/19 00:00 [accepted]
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2013/11/06 06:00 [medline]
AID - 10.3892/mmr.2013.1396 [doi]
PST - ppublish
SO  - Mol Med Rep. 2013 May;7(5):1607-12. doi: 10.3892/mmr.2013.1396. Epub 2013 Mar 26.