PMID- 23546950
OWN - NLM
STAT- MEDLINE
DCOM- 20130920
LR  - 20130402
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 12
IP  - 1
DP  - 2013 Mar 11
TI  - Molecular cytogenetic characterization of the Aegilops biuncialis karyotype.
PG  - 683-92
LID - 10.4238/2013.March.11.16 [doi]
AB  - Aegilops biuncialis can be hybridized with wheat (Triticum spp) and has been used 
      for wheat breeding and genetic studies. The A. biuncialis karyotype (U(b) U(b) 
      M(b) M(b)) was investigated based on three A. biuncialis accessions grown in 
      China. Two pairs of SAT chromosomes were identified as 1U(b) and 5U(b), with a 
      karyotype formula of 2n = 4x = 28 = 14m + 10sm + 4st. Fluorescence in situ 
      hybridization (FISH) and C-banding approaches were used to analyze the A. 
      biuncialis accession chromosomes at the mitotic stage. Based on the C-banding and 
      FISH patterns, all U(b) and M(b) chromosomes could be discriminated 
      simultaneously; the three A. biuncialis accessions exhibited similar patterns, 
      suggesting a common origin. The U(b) genome from A. biuncialis resembled the U 
      genome in the diploid species A. umbellulata, and it may be related to the 
      tetraploid species containing the U genome. The M(b) genome had some differences 
      compared to the M genome in the diploid species A. comosa, and it may be related 
      to the tetraploid species possessing the M genome. A generalized ideogram was 
      proposed for the A. biuncialis genome, which could be useful for standardized and 
      accurate identification of the A. biuncialis karyotype and chromosomes.
FAU - Wang, J
AU  - Wang J
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.
FAU - Zhang, W
AU  - Zhang W
FAU - Zhao, H
AU  - Zhao H
FAU - Li, F R
AU  - Li FR
FAU - Wang, Z G
AU  - Wang ZG
FAU - Ji, J
AU  - Ji J
FAU - Zhang, X Q
AU  - Zhang XQ
FAU - Wang, D W
AU  - Wang DW
FAU - Li, J M
AU  - Li JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130311
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
SB  - IM
MH  - Chromosome Banding
MH  - Chromosomes, Plant/*genetics
MH  - Diploidy
MH  - Genome, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Karyotyping/*methods
MH  - Poaceae/classification/*genetics
MH  - Polyploidy
MH  - Species Specificity
EDAT- 2013/04/03 06:00
MHDA- 2013/09/21 06:00
CRDT- 2013/04/03 06:00
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
AID - gmr2119 [pii]
AID - 10.4238/2013.March.11.16 [doi]
PST - epublish
SO  - Genet Mol Res. 2013 Mar 11;12(1):683-92. doi: 10.4238/2013.March.11.16.