PMID- 23547051
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20211021
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 121
IP  - 20
DP  - 2013 May 16
TI  - C/EBPalpha is required for development of dendritic cell progenitors.
PG  - 4073-81
LID - 10.1182/blood-2012-10-463448 [doi]
AB  - Dendritic cells (DCs) are master regulators of the immune system, but molecular 
      regulation of early DC differentiation has been poorly understood. Here, we 
      report that the transcription factor C/EBPalpha coordinates the development of 
      progenitor cells required for production of multiple categories of DCs. C/EBPalpha 
      was needed for differentiation from stem/progenitor cells to common DC 
      progenitors (CDPs), but not for transition of CDP to mature DCs. C/EBPalpha deletion 
      in mature DCs did not affect their numbers or function, suggesting that this 
      transcription factor is not needed for maintenance of DCs in lymphoid tissues. 
      ChIP-seq and microarrays were used to identify candidate genes regulated by 
      C/EBPalpha and required for DC formation. Genes previously shown to be critical for 
      DC formation were bound by C/EBPalpha, and their expression was decreased in the 
      earliest hematopoietic compartments in the absence of C/EBPalpha. These data indicate 
      that C/EBPalpha is important for the earliest stages of steady-state DC 
      differentiation.
FAU - Welner, Robert S
AU  - Welner RS
AD  - Harvard Stem Cell Institute, Harvard Medical School, Boston, MA, USA.
FAU - Bararia, Deepak
AU  - Bararia D
FAU - Amabile, Giovanni
AU  - Amabile G
FAU - Czibere, Akos
AU  - Czibere A
FAU - Benoukraf, Touati
AU  - Benoukraf T
FAU - Bach, Christian
AU  - Bach C
FAU - Wansa, K Disnika Senali Abayratna
AU  - Wansa KD
FAU - Ye, Min
AU  - Ye M
FAU - Zhang, Hong
AU  - Zhang H
FAU - Iino, Tadafumi
AU  - Iino T
FAU - Hetherington, Christopher J
AU  - Hetherington CJ
FAU - Akashi, Koichi
AU  - Akashi K
FAU - Tenen, Daniel G
AU  - Tenen DG
LA  - eng
GR  - CA66996/CA/NCI NIH HHS/United States
GR  - P01 DK080665/DK/NIDDK NIH HHS/United States
GR  - R01 CA118316/CA/NCI NIH HHS/United States
GR  - CA118316/CA/NCI NIH HHS/United States
GR  - P01 CA066996/CA/NCI NIH HHS/United States
GR  - R01 CA041456/CA/NCI NIH HHS/United States
GR  - R01 HL112719/HL/NHLBI NIH HHS/United States
GR  - DK080665/DK/NIDDK NIH HHS/United States
GR  - HL56745/HL/NHLBI NIH HHS/United States
GR  - CA41456/CA/NCI NIH HHS/United States
GR  - R01 HL056745/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130401
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
SB  - IM
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics/metabolism/*physiology
MH  - Cell Differentiation/*genetics/immunology
MH  - Cells, Cultured
MH  - Cluster Analysis
MH  - Dendritic Cells/metabolism/*physiology
MH  - Gene Expression Profiling
MH  - Gene Knockdown Techniques
MH  - Lymphoid Tissue/cytology/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - Oligonucleotide Array Sequence Analysis
MH  - Stem Cells/metabolism/*physiology
PMC - PMC3656447
EDAT- 2013/04/03 06:00
MHDA- 2013/08/06 06:00
PMCR- 2014/05/16
CRDT- 2013/04/03 06:00
PHST- 2013/04/03 06:00 [entrez]
PHST- 2013/04/03 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
PHST- 2014/05/16 00:00 [pmc-release]
AID - S0006-4971(20)58495-4 [pii]
AID - 2012/463448 [pii]
AID - 10.1182/blood-2012-10-463448 [doi]
PST - ppublish
SO  - Blood. 2013 May 16;121(20):4073-81. doi: 10.1182/blood-2012-10-463448. Epub 2013 
      Apr 1.