PMID- 23547372
OWN - NLM
STAT- MEDLINE
DCOM- 20130613
LR  - 20161021
IS  - 1000-8721 (Print)
IS  - 1000-8721 (Linking)
VI  - 29
IP  - 1
DP  - 2013 Jan
TI  - [Identification of Nam Dinh virus in China].
PG  - 1-6
AB  - During 2009-2012, the Nam Dinh virus (NDiv) was detected from the samples of 
      Culex pipiens quinquefasciatus in Shenzhen China. In this study, cell 
      culture,SYBR Green I based real time RT-PCR and RT-PCR were performed to analyze 
      the cell susceptibility and other biological characteristics of the NDiV 
      isolates. The results showed that C6/36 cell line was susceptible to four 
      isolates of Culex pipiens quinquefasciatus. The "S" type amplification curve and 
      specific melting curve were obtained in the realtime fluorescence quantitative 
      RT-PCR based on SYBR Green I for the detection of the NDiV from the mosquito. The 
      target bands from the RdRp gene and partial fragment of ZmHel1 gene were observed 
      using agarose gel electrophoresis. Both the nucleotide and amino acid sequences 
      of four Shenzhen isolates showed more than 99.00% homology with the Vietnam 
      representative NDiV strain (02VN178). Phylogenetic analysis showed that four 
      Shenzhen isolates shared the same evolution branch as the Vietnam representative 
      NDiV strain. This is the first report of NDiV in China.
FAU - Liu, Qu
AU  - Liu Q
AD  - Longgang Center for Disease Control and Prevention of Shenzhen, Shenzhen 518172, 
      China. szliuqu@163.com
FAU - Lin, Lin
AU  - Lin L
FAU - Zhou, Jian-Ming
AU  - Zhou JM
FAU - Chen, Ying-Jian
AU  - Chen YJ
FAU - Zhang, Qi-Wen
AU  - Zhang QW
FAU - Wang, De-Quan
AU  - Wang DQ
FAU - Li, Jing-Mei
AU  - Li JM
FAU - Jin, Yu-Juan
AU  - Jin YJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Bing Du Xue Bao
JT  - Bing du xue bao = Chinese journal of virology
JID - 8803009
SB  - IM
MH  - Animals
MH  - China
MH  - Culex/*virology
MH  - Nidovirales/classification/genetics/*isolation & purification
MH  - Phylogeny
EDAT- 2013/04/04 06:00
MHDA- 2013/06/14 06:00
CRDT- 2013/04/04 06:00
PHST- 2013/04/04 06:00 [entrez]
PHST- 2013/04/04 06:00 [pubmed]
PHST- 2013/06/14 06:00 [medline]
PST - ppublish
SO  - Bing Du Xue Bao. 2013 Jan;29(1):1-6.