PMID- 23548744
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20211021
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 25
IP  - 4
DP  - 2013 Apr
TI  - PHYTOCHROME INTERACTING FACTOR3 associates with the histone deacetylase HDA15 in 
      repression of chlorophyll biosynthesis and photosynthesis in etiolated 
      Arabidopsis seedlings.
PG  - 1258-73
LID - 10.1105/tpc.113.109710 [doi]
AB  - PHYTOCHROME INTERACTING FACTOR3 (PIF3) is a key basic helix-loop-helix 
      transcription factor of Arabidopsis thaliana that negatively regulates light 
      responses, repressing chlorophyll biosynthesis, photosynthesis, and 
      photomorphogenesis in the dark. However, the mechanism for the PIF3-mediated 
      transcription regulation remains largely unknown. In this study, we found that 
      the REDUCED POTASSIUM DEPENDENCY3/HISTONE DEACETYLASE1-type histone deacetylase 
      HDA15 directly interacted with PIF3 in vivo and in vitro. Genome-wide 
      transcriptome analysis revealed that HDA15 acts mainly as a transcriptional 
      repressor and negatively regulates chlorophyll biosynthesis and photosynthesis 
      gene expression in etiolated seedlings. HDA15 and PIF3 cotarget to the genes 
      involved in chlorophyll biosynthesis and photosynthesis in the dark and repress 
      gene expression by decreasing the acetylation levels and RNA Polymerase 
      II-associated transcription. The binding of HDA15 to the target genes depends on 
      the presence of PIF3. In addition, PIF3 and HDA15 are dissociated from the target 
      genes upon exposure to red light. Taken together, our results indicate that PIF3 
      associates with HDA15 to repress chlorophyll biosynthetic and photosynthetic 
      genes in etiolated seedlings.
FAU - Liu, Xuncheng
AU  - Liu X
AD  - Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
FAU - Chen, Chia-Yang
AU  - Chen CY
FAU - Wang, Ko-Ching
AU  - Wang KC
FAU - Luo, Ming
AU  - Luo M
FAU - Tai, Ready
AU  - Tai R
FAU - Yuan, Lianyu
AU  - Yuan L
FAU - Zhao, Minglei
AU  - Zhao M
FAU - Yang, Songguang
AU  - Yang S
FAU - Tian, Gang
AU  - Tian G
FAU - Cui, Yuhai
AU  - Cui Y
FAU - Hsieh, Hsu-Liang
AU  - Hsieh HL
FAU - Wu, Keqiang
AU  - Wu K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130402
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (PIF3 protein, Arabidopsis)
RN  - 1406-65-1 (Chlorophyll)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.5.1.98 (HDA15 protein, Arabidopsis)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Arabidopsis/*genetics/growth & development/metabolism
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics/metabolism
MH  - Cell Nucleus/metabolism
MH  - Chlorophyll/*biosynthesis
MH  - Etiolation/genetics
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Plant
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Histone Deacetylases/*genetics/metabolism
MH  - Hypocotyl/genetics/growth & development/metabolism
MH  - Models, Genetic
MH  - Photosynthesis/*genetics
MH  - Plants, Genetically Modified
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seedlings/*genetics/growth & development/metabolism
MH  - Transcriptome/genetics
PMC - PMC3663266
EDAT- 2013/04/04 06:00
MHDA- 2014/01/01 06:00
PMCR- 2013/04/02
CRDT- 2013/04/04 06:00
PHST- 2013/04/04 06:00 [entrez]
PHST- 2013/04/04 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2013/04/02 00:00 [pmc-release]
AID - tpc.113.109710 [pii]
AID - 109710 [pii]
AID - 10.1105/tpc.113.109710 [doi]
PST - ppublish
SO  - Plant Cell. 2013 Apr;25(4):1258-73. doi: 10.1105/tpc.113.109710. Epub 2013 Apr 2.