PMID- 23548949
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20130618
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 161
IP  - 3
DP  - 2013
TI  - Sublingual immunotherapy for experimental allergic conjunctivitis in a murine 
      model induced by Dermatophagoides farinae allergen.
PG  - 205-12
LID - 10.1159/000346335 [doi]
AB  - BACKGROUND: Sublingual immunotherapy (SLIT) is a clinically effective treatment 
      in allergic conjunctivitis (AC); however, the mechanism of the underlying 
      pharmacodynamics remains unclear. Here, we investigate the efficacy and the 
      mechanism of a sublingually administered Dermatophagoides farinae (Der f) vaccine 
      in a murine AC model. METHODS: A murine model of AC caused by Der f extract was 
      developed in BALB/c mice by repeated application of allergen. Sensitized mice 
      were SLIT treated by Der f drops and subsequently analyzed for AC symptoms, 
      histopathological and immunological parameters. RESULTS: In this study, Der f 
      extract successfully induced the symptoms of AC in BALB/c mice. In these 
      sensitized mice, clinical symptoms (scratching behavior, lacrimation, 
      conjunctival hyperemia and edema), immunological and histopathological findings 
      (inflammatory cell infiltration) were very similar to those in human AC. SLIT 
      treatment of sensitized mice markedly reduced the clinical and histopathological 
      symptoms and decreased the expression levels of total immunoglobulin E (IgE), Der 
      f-specific IgE and T helper cell 2 (Th2) cytokine interleukin-4, with a 
      significant increase in Der f-specific IgG4 and Th1 cytokine interferon-gamma. 
      CONCLUSIONS: SLIT with Der f drops is a potentially effective means of 
      immunotherapy for Der f-induced AC by modulating the Th2-biased allergic immune 
      response.
CI  - Copyright (c) 2013 S. Karger AG, Basel.
FAU - Liu, Li-lin
AU  - Liu LL
AD  - Division of Research and Development, Wolwo Bio-Pharmaceutical Inc., Shanghai, PR 
      China.
FAU - Guo, Dan-dan
AU  - Guo DD
FAU - Liang, Qiao-xia
AU  - Liang QX
FAU - Ding, Shan
AU  - Ding S
FAU - Chen, Jing-ya
AU  - Chen JY
FAU - Wu, Bing
AU  - Wu B
FAU - Li, Qin
AU  - Li Q
LA  - eng
PT  - Journal Article
DEP - 20130315
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides farinae antigen f 1)
SB  - IM
MH  - Administration, Sublingual
MH  - Animals
MH  - Anti-Allergic Agents/therapeutic use
MH  - Antigens, Dermatophagoides/immunology/*therapeutic use
MH  - Arthropod Proteins/immunology/*therapeutic use
MH  - Conjunctiva/pathology
MH  - Conjunctivitis, Allergic/immunology/*therapy
MH  - Cysteine Endopeptidases/immunology/*therapeutic use
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - *Immunotherapy, Active
MH  - Interleukin-4/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Th2 Cells/cytology
EDAT- 2013/04/04 06:00
MHDA- 2013/09/14 06:00
CRDT- 2013/04/04 06:00
PHST- 2012/07/12 00:00 [received]
PHST- 2012/12/05 00:00 [accepted]
PHST- 2013/04/04 06:00 [entrez]
PHST- 2013/04/04 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
AID - 000346335 [pii]
AID - 10.1159/000346335 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2013;161(3):205-12. doi: 10.1159/000346335. Epub 2013 
      Mar 15.