PMID- 23549419
OWN - NLM
STAT- MEDLINE
DCOM- 20131031
LR  - 20181202
IS  - 1523-1747 (Electronic)
IS  - 0022-202X (Linking)
VI  - 133
IP  - 9
DP  - 2013 Sep
TI  - Keratinocyte stem cells but not melanocyte stem cells are the primary target for 
      radiation-induced hair graying.
PG  - 2143-51
LID - 10.1038/jid.2013.155 [doi]
AB  - Ionizing radiation (IR)-induced hair graying is caused by the ectopic 
      differentiation of melanocyte stem cells (MSCs) in their niche located at the 
      bulge region of the hair follicle. Keratinocyte stem cells (KSCs) in the bulge 
      region are an important component of that niche. However, little is known about 
      the relationship between MSC differentiation and the KSC niche during IR-induced 
      hair graying. We found that both follicular MSCs and KSCs were affected by IR by 
      using immunohistochemical detection of gammaH2AX as a genotoxicity marker. We also 
      found that KSCs prepared from irradiated mice were functionally affected by IR as 
      indicated by their reduced colony-forming activity in culture and the delayed 
      hair cycle in vivo. However, these effects of IR on KSCs were temporal. The MSC 
      population, which proliferated and differentiated to melanocytes, was 
      persistently maintained after irradiation. In addition to the loss of 
      colony-forming activity, irradiated keratinocytes including KSCs suppressed the 
      colony formation of MSCs in vitro. Furthermore, pigmented hairs were not 
      reconstituted in vivo in the presence of irradiated KSCs or keratinocytes. These 
      results provide a previously unreported insight that the primary target of IR 
      during the induction of hair graying is follicular KSCs rather than MSCs.
FAU - Aoki, Hitomi
AU  - Aoki H
AD  - Department of Tissue and Organ Development, Regeneration, and Advanced Medical 
      Science, Gifu, Japan.
FAU - Hara, Akira
AU  - Hara A
FAU - Motohashi, Tsutomu
AU  - Motohashi T
FAU - Kunisada, Takahiro
AU  - Kunisada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130402
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
SB  - IM
MH  - Adult Stem Cells/cytology/*radiation effects
MH  - Animals
MH  - Cell Differentiation/radiation effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - DNA Damage/radiation effects
MH  - Epidermal Cells
MH  - Epidermis/radiation effects
MH  - Hair Color/*radiation effects
MH  - Hair Follicle/cytology/radiation effects
MH  - Keratinocytes/cytology/*radiation effects
MH  - Lac Operon
MH  - Melanocytes/cytology/*radiation effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Regeneration/radiation effects
MH  - Whole-Body Irradiation/*methods
EDAT- 2013/04/04 06:00
MHDA- 2013/11/01 06:00
CRDT- 2013/04/04 06:00
PHST- 2012/11/20 00:00 [received]
PHST- 2013/02/27 00:00 [revised]
PHST- 2013/03/10 00:00 [accepted]
PHST- 2013/04/04 06:00 [entrez]
PHST- 2013/04/04 06:00 [pubmed]
PHST- 2013/11/01 06:00 [medline]
AID - S0022-202X(15)36411-3 [pii]
AID - 10.1038/jid.2013.155 [doi]
PST - ppublish
SO  - J Invest Dermatol. 2013 Sep;133(9):2143-51. doi: 10.1038/jid.2013.155. Epub 2013 
      Apr 2.