PMID- 23553963
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20181202
IS  - 1552-8618 (Electronic)
IS  - 0730-7268 (Linking)
VI  - 32
IP  - 7
DP  - 2013 Jul
TI  - CYP1A expression in liver and gills of roach (Rutilus rutilus) after waterborne 
      exposure to two phenanthrene derivatives, 1-methylphenanthrene and 
      4-methylphenanthrene.
PG  - 1604-10
LID - 10.1002/etc.2225 [doi]
AB  - Phenanthrenes (Phs) substituted with alkyl groups are a class of compound present 
      in the environment, and they appear to be toxic to developing fish. The present 
      study aimed to investigate the effect of waterborne exposure to two monomethyl 
      derivatives of phenanthrene, 1-methylphenanthrene (1M-Ph) and 
      4-methylphenanthrene (4M-Ph), on cytochrome P450 1A (CYP1A) gene expression in 
      fish gills and liver. Juvenile common roaches (Rutilus rutilus) were exposed to 
      water with dimethyl sulfoxide (DMSO) solutions of 1M-Ph, 4M-Ph, benzo[a]pyrene 
      (BaP; positive control), each at a dose of 100 microg/L, or to water with DMSO alone 
      (negative control group) for 2 d and 7 d. Significant CYP1A responses with regard 
      to treatment and exposure duration were noted (2-way analysis of variance 
      [ANOVA]) in gills (p = 0.013 and p = 0.003, respectively) and liver (p < 0.001). 
      The 2 monomethyl Phs did not induce consistent gene expression changes, except 
      for 4-MPh, which elevated the CYP1A messenger ribonucleic acid (mRNA) level in 
      the liver at the end of the treatment (almost 4-fold; p < 0.05; 7 d). As was 
      expected, exposure to BaP resulted in elevation of CYP1A mRNA expression in 
      treated fish compared with the control group. Expressions after 2 d and 7 d were 
      approximately 220- and 180-fold higher in liver and 8- and 6-fold higher in gills 
      respectively. The CYP1A protein levels remained stable in both tissues, with one 
      notable exception in roach liver treated for 2 d with BaP ( approximately  6-fold increase; 
      p < 0.05). The different effects of the 1- and 4-methylphenanthrenes on CYP1A 
      gene expression in roach liver suggest a relationship between chemical or 3-D 
      structure of the differentially substituted monomethyl Phs and their biological 
      activity.
CI  - Copyright (c) 2013 SETAC.
FAU - Wolinska, Lidia
AU  - Wolinska L
AD  - Department of Environmental Biotechnology, Faculty of Environmental Sciences, 
      University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. 
      lidia.wolinska@uwm.edu.pl
FAU - Brzuzan, Pawel
AU  - Brzuzan P
FAU - Wozny, Maciej
AU  - Wozny M
FAU - Luczynski, Michal K
AU  - Luczynski MK
FAU - Gora, Maciej
AU  - Gora M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130520
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Phenanthrenes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 44IY90KLCE (1-methylphenanthrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Cyprinidae/genetics/metabolism
MH  - Cytochrome P-450 CYP1A1/genetics/metabolism
MH  - Gene Expression/drug effects
MH  - Gills/drug effects/metabolism
MH  - Liver/drug effects/metabolism
MH  - Phenanthrenes/metabolism/*toxicity
MH  - RNA, Messenger/metabolism
MH  - Water Pollutants, Chemical/metabolism/*toxicity
EDAT- 2013/04/05 06:00
MHDA- 2013/08/02 06:00
CRDT- 2013/04/05 06:00
PHST- 2012/11/05 00:00 [received]
PHST- 2012/12/17 00:00 [revised]
PHST- 2013/03/13 00:00 [accepted]
PHST- 2013/04/05 06:00 [entrez]
PHST- 2013/04/05 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
AID - 10.1002/etc.2225 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2013 Jul;32(7):1604-10. doi: 10.1002/etc.2225. Epub 2013 
      May 20.