PMID- 23554017
OWN - NLM
STAT- MEDLINE
DCOM- 20140221
LR  - 20130828
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Linking)
VI  - 67
IP  - 10
DP  - 2013 Oct
TI  - Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve 
      regeneration with acellular nerve allografts.
PG  - 637-47
LID - 10.1002/syn.21669 [doi]
AB  - Acellular nerve allografts (ANA) possess bioactivity and neurite promoting 
      factors in nerve tissue engineering. Previously we reported that low dose 
      ultrashort wave (USW) radiation could enhance the rate and quality of peripheral 
      nerve regeneration with ANA repairing sciatic nerve defects. Meanwhile, ANA 
      implanted with bone marrow stromal cells (BMSCs) exhibited a similar result. 
      Thus, it is interesting to know whether it might yield a synergistic effect when 
      USW radiation is combined with BMSCs-laden ANA. Here we investigated the 
      effectiveness of ANA seeded with BMSCs, combined with USW therapy on repairing 
      peripheral nerve injuries. Adult male Wistar rats were randomly divided into four 
      groups: Dulbecco's modified Eagle's medium (DMEM) control group, BMSCs-laden 
      group, ultrashort wave (USW) group and BMSC + USW group. The regenerated nerves 
      were assayed morphologically and functionally, and growth-promoting factors in 
      the regenerated tissues following USW administration or BMSCs integration were 
      also detected. The results indicated that the combination therapy caused much 
      better beneficial effects evidenced by increased myelinated nerve fiber number, 
      myelin sheath thickness, axon diameter, sciatic function index, nerve conduction 
      velocity, and restoration rate of tibialis anterior wet weight. Moreover, the 
      mRNA levels of brain-derived neurotrophic factor (BDNF) and vascular endothelial 
      growth factor (VEGF) in the spinal cord and muscles were elevated significantly. 
      In conclusion, we found a synergistic effect of USW radiation and BMSCs treatment 
      on peripheral nerve regeneration, which may help establish novel strategies for 
      repairing peripheral nerve defects.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Pang, Chao-Jian
AU  - Pang CJ
AD  - Department of Anatomy, College of Basic Medical Sciences, China Medical 
      University, Shenyang, China.
FAU - Tong, Lei
AU  - Tong L
FAU - Ji, Li-Li
AU  - Ji LL
FAU - Wang, Zhen-Yu
AU  - Wang ZY
FAU - Zhang, Xu
AU  - Zhang X
FAU - Gao, Hai
AU  - Gao H
FAU - Jia, Hua
AU  - Jia H
FAU - Zhang, Li-Xin
AU  - Zhang LX
FAU - Tong, Xiao-Jie
AU  - Tong XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130502
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Combined Modality Therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Myelin Sheath/metabolism
MH  - *Nerve Regeneration
MH  - Neural Conduction
MH  - Peripheral Nerve Injuries/*therapy
MH  - Peripheral Nerves/*transplantation
MH  - Rats
MH  - Rats, Wistar
MH  - Sciatic Nerve/injuries/metabolism/*physiology/radiation effects
MH  - *Short-Wave Therapy
MH  - Transplantation, Homologous
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - Schwann cells
OT  - bone marrow stromal cells
OT  - peripheral nerve regeneration
OT  - ultrashort wave therapy
EDAT- 2013/04/05 06:00
MHDA- 2014/02/22 06:00
CRDT- 2013/04/05 06:00
PHST- 2012/10/16 00:00 [received]
PHST- 2013/01/07 00:00 [revised]
PHST- 2013/03/22 00:00 [accepted]
PHST- 2013/04/05 06:00 [entrez]
PHST- 2013/04/05 06:00 [pubmed]
PHST- 2014/02/22 06:00 [medline]
AID - 10.1002/syn.21669 [doi]
PST - ppublish
SO  - Synapse. 2013 Oct;67(10):637-47. doi: 10.1002/syn.21669. Epub 2013 May 2.