PMID- 23554258
OWN - NLM
STAT- MEDLINE
DCOM- 20131213
LR  - 20240322
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 64
IP  - 8
DP  - 2013 May
TI  - Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei 
      reveals a strong distal bias and weak interference.
PG  - 2139-54
LID - 10.1093/jxb/ert079 [doi]
AB  - In barley (Hordeum vulgare L.), chiasmata (the physical sites of genetic 
      crossovers) are skewed towards the distal ends of chromosomes, effectively 
      consigning a large proportion of genes to recombination coldspots. This has the 
      effect of limiting potential genetic variability, and of reducing the efficiency 
      of map-based cloning and breeding approaches for this crop. Shifting the sites of 
      recombination to more proximal chromosome regions by forward and reverse genetic 
      means may be profitable in terms of realizing the genetic potential of the 
      species, but is predicated upon a better understanding of the mechanisms 
      governing the sites of these events, and upon the ability to recognize real 
      changes in recombination patterns. The barley MutL Homologue (HvMLH3), a marker 
      for class I interfering crossovers, has been isolated and a specific antibody has 
      been raised. Immunolocalization of HvMLH3 along with the synaptonemal complex 
      transverse filament protein ZYP1, used in conjunction with fluorescence in situ 
      hybridization (FISH) tagging of specific barley chromosomes, has enabled access 
      to the physical recombination landscape of the barley cultivars Morex and Bowman. 
      Consistent distal localization of HvMLH3 foci throughout the genome, and similar 
      patterns of HvMLH3 foci within bivalents 2H and 3H have been observed. A 
      difference in total numbers of HvMLH3 foci between these two cultivars has been 
      quantified, which is interpreted as representing genotypic variation in class I 
      crossover frequency. Discrepancies between the frequencies of HvMLH3 foci and 
      crossover frequencies derived from linkage analysis point to the existence of at 
      least two crossover pathways in barley. It is also shown that interference of 
      HvMLH3 foci is relatively weak compared with other plant species.
FAU - Phillips, Dylan
AU  - Phillips D
AD  - Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Penglais, Aberystwyth, Ceredigion SY23 3DA, UK.
FAU - Wnetrzak, Joanna
AU  - Wnetrzak J
FAU - Nibau, Candida
AU  - Nibau C
FAU - Barakate, Abdellah
AU  - Barakate A
FAU - Ramsay, Luke
AU  - Ramsay L
FAU - Wright, Frank
AU  - Wright F
FAU - Higgins, James D
AU  - Higgins JD
FAU - Perry, Ruth M
AU  - Perry RM
FAU - Jenkins, Glyn
AU  - Jenkins G
LA  - eng
SI  - GENBANK/JQ855501
GR  - BB/F019351/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/F020872/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/F018754/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130403
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
SB  - IM
MH  - Arabidopsis/genetics
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Plant/*genetics/physiology
MH  - Crossing Over, Genetic/genetics/physiology
MH  - Genetic Linkage/genetics/physiology
MH  - Genetic Loci/genetics/physiology
MH  - Genome, Plant/genetics/physiology
MH  - Hordeum/*genetics/physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Pachytene Stage/*genetics/physiology
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Synaptonemal Complex/genetics/physiology
PMC - PMC3654414
OTO - NOTNLM
OT  - Barley
OT  - Hordeum vulgare
OT  - MLH3.
OT  - chiasma
OT  - crossover
OT  - interference
EDAT- 2013/04/05 06:00
MHDA- 2013/12/18 06:00
PMCR- 2013/04/03
CRDT- 2013/04/05 06:00
PHST- 2013/04/05 06:00 [entrez]
PHST- 2013/04/05 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PHST- 2013/04/03 00:00 [pmc-release]
AID - ert079 [pii]
AID - 10.1093/jxb/ert079 [doi]
PST - ppublish
SO  - J Exp Bot. 2013 May;64(8):2139-54. doi: 10.1093/jxb/ert079. Epub 2013 Apr 3.