PMID- 23554644
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130405
LR  - 20211021
IS  - 1674-8301 (Print)
IS  - 1674-8301 (Linking)
VI  - 24
IP  - 4
DP  - 2010 Jul
TI  - Activation of calcium-sensing receptors is associated with apoptosis in a model 
      of simulated cardiomyocytes ischemia/reperfusion.
PG  - 301-7
LID - 10.1016/S1674-8301(10)60042-5 [doi]
AB  - OBJECTIVE: Calcium-sensing receptors (CaSRs) are G-protein coupled receptors 
      which maintain systemic calcium homeostasis and participate in hormone secretion, 
      activation of ion channels, cell apoptosis, proliferation, and differentiation. 
      Previous studies have shown that CaSRs induce apoptosis in isolated adult rat 
      heart and in normal neonatal rat cardiomyocytes by G-protein-PLC-IP3 signaling 
      transduction. However, little knowledge is presently available concerning the 
      role of CaSRs in the apoptosis induced by ischemia and reperfusion in neonatal 
      cardiomyocytes. METHODS: Primary neonatal rat ventricular cardiomyocytes were 
      incubated in ischemiamimetic solution for 2 h, and then re-incubated in normal 
      culture medium for 24 h to establish a model of simulated ischemia/reperfusion 
      (I/R). Cardiomyocyte apoptosis was detected by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL). The expression of CaSRs mRNA 
      was detected by real-time reverse transcription polymerase chain reaction 
      (RT-PCR). In addition, the expressions of caspase-3 and Bcl-2 were analyzed by 
      western blot. RESULTS: The simulated I/R enhanced the expression of CaSRs and 
      cardiomyocyte apoptosis. GdCl3, a specific activator of CaSRs, further increased 
      the expression of CaSRs and cardiomyocyte apoptosis, along with up-regulation of 
      caspase-3 and down-regulation of Bcl-2. CONCLUSION: CaSRs are associated with I/R 
      injury and apoptosis in neonatal rat ventricular cardiomyocytes via suppressing 
      Bcl-2 and promoting caspase-3 expression.
FAU - Yan, Ling
AU  - Yan L
AD  - Department of Cardiology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, Jiangsu Province, China.
FAU - Zhu, Tiebing
AU  - Zhu T
FAU - Sun, Tingting
AU  - Sun T
FAU - Wang, Liansheng
AU  - Wang L
FAU - Pan, Shiyang
AU  - Pan S
FAU - Tao, Zhengxian
AU  - Tao Z
FAU - Yang, Zhijian
AU  - Yang Z
FAU - Cao, Kejiang
AU  - Cao K
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Biomed Res
JT  - Journal of biomedical research
JID - 101551157
PMC - PMC3596596
OTO - NOTNLM
OT  - apoptosis
OT  - calcium sensing receptors
OT  - cardiomyocyte
OT  - ischemia/reperfusion
EDAT- 2010/07/01 00:00
MHDA- 2010/07/01 00:01
PMCR- 2010/07/01
CRDT- 2013/04/05 06:00
PHST- 2009/11/25 00:00 [received]
PHST- 2013/04/05 06:00 [entrez]
PHST- 2010/07/01 00:00 [pubmed]
PHST- 2010/07/01 00:01 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - jbr-24-04-301 [pii]
AID - 10.1016/S1674-8301(10)60042-5 [doi]
PST - ppublish
SO  - J Biomed Res. 2010 Jul;24(4):301-7. doi: 10.1016/S1674-8301(10)60042-5.