PMID- 23556537
OWN - NLM
STAT- MEDLINE
DCOM- 20150128
LR  - 20140414
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 25
IP  - 3
DP  - 2014
TI  - Detection of expression of IL-18 and its binding protein in Egyptian pediatric 
      immune thrombocytopenic purpura.
PG  - 193-6
LID - 10.3109/09537104.2013.784734 [doi]
AB  - Immune thrombocytopenic purpura (ITP) is an autoimmune disorder, characterized by 
      dysfunctional cellular immunity including the presence of activated platelet 
      specific autoreactive T cells that recognize and respond to autologous platelet 
      antigens. Autoreactive T cells drive the generation of platelet reactive 
      autoantibodies by B cells as well as T-cytotoxic cell-mediated lysis of 
      platelets. Interleukin-18 (IL-18) is a mediator of T helper type 1 cell responses 
      synergistically with IL-12 that initiate and promote host defense and 
      inflammation. IL-18 has a specific binding protein (IL-18BP) which belongs to the 
      immunoglobulin superfamily. In the present study, serum level and messenger RNA( 
      mRNA) expression of IL-18 as well as IL-18BP mRNA expression were measured in 
      peripheral blood mononuclear cells (PBMNCs) of 100 Egyptian pediatric patients 
      with ITP (70 acute and 30 chronic). In addition to this, we recruited 80 healthy 
      volunteers in order to investigate the possible association between the imbalance 
      of IL-18 and IL-18 BP expressions and the pathogenesis of ITP. IL-18 serum level 
      and mRNA expression were not elevated in cases more than in the control group, 
      but IL-18 mRNA was higher in chronic cases when compared to the acute ones 
      (p=0.031) and there was a good negative correlation between the platelet count 
      and serum IL-18. IL-18 BP m-RNA was slightly elevated in cases more than in the 
      control group (95% Confidence interval=1.15-2.01). Our results were not 
      supportive for previous findings of elevated IL18/BP mRNA ratio in ITP patients. 
      This could be referred to the fact that autoimmune diseases are complex genetic 
      disorders, therefore further studies on polymorphisms affecting IL-18 gene 
      expression as well as kinetics of IL-18 expression are required to evaluate the 
      role of interleukin 18 and its binding protein in the pathogenesis of ITP.
FAU - Shaheen, Iman A
AU  - Shaheen IA
AD  - Department of Clinical Pathology, Cairo University , Cairo , Egypt and.
FAU - Botros, Shahira K A
AU  - Botros SK
FAU - Morgan, Dalia S
AU  - Morgan DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130404
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 0 (interleukin-18 binding protein)
SB  - IM
MH  - Adolescent
MH  - Blood Platelets/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Egypt
MH  - Female
MH  - Humans
MH  - Infant
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis/blood/genetics
MH  - Interleukin-18/*biosynthesis/blood/genetics
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Purpura, Thrombocytopenic, Idiopathic/*blood/genetics
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2013/04/06 06:00
MHDA- 2015/01/30 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
AID - 10.3109/09537104.2013.784734 [doi]
PST - ppublish
SO  - Platelets. 2014;25(3):193-6. doi: 10.3109/09537104.2013.784734. Epub 2013 Apr 4.