PMID- 23557133
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20240420
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 22
IP  - 17
DP  - 2013 Sep 1
TI  - Fibroblast growth factor-1 is a mesenchymal stromal cell-secreted factor 
      stimulating proliferation of osteoarthritic chondrocytes in co-culture.
PG  - 2356-67
LID - 10.1089/scd.2013.0118 [doi]
AB  - Previously, we showed that mesenchymal stromal cells (MSCs) in co-culture with 
      primary chondrocytes secrete soluble factors that increase chondrocyte 
      proliferation. The objective of this study is to identify these factors. Human 
      primary chondrocytes (hPCs) isolated from late-stage osteoarthritis patients were 
      co-cultured with human bone marrow-derived MSCs (hMSCs) in pellets. Genome-wide 
      mRNA expression analysis and quantitative polymerase chain reactions (qPCR) were 
      used to identify soluble factors that were specifically induced in co-cultures. 
      Immunofluorescent staining combined with cell tracking and enzyme-linked 
      immunosorbent assay (ELISA) were performed to validate up-regulation at the 
      protein level and to identify the cellular origin of the increased proteins. 
      Chemical blockers and neutralizing antibodies were used to elucidate the role of 
      the identified candidate genes in co-cultures. A number of candidate factors were 
      differentially regulated in co-cultures at the mRNA level. Of these, fibroblast 
      growth factor-1 (FGF-1) mRNA and protein expression were markedly increased in 
      co-cultures predominantly due to up-regulated expression in MSCs. Blocking of FGF 
      signaling in co-culture pellets by specific FGF receptor inhibitors or FGF-1 
      neutralizing antibodies completely blocked hPCs proliferation. We demonstrate 
      that MSCs increase FGF-1 secretion on co-culture with hPCs, which, in turn, is 
      responsible for increased hPCs proliferation in pellet co-cultures.
FAU - Wu, Ling
AU  - Wu L
AD  - Department of Developmental BioEngineering, University of Twente , Enschede, The 
      Netherlands.
FAU - Leijten, Jeroen
AU  - Leijten J
FAU - van Blitterswijk, Clemens A
AU  - van Blitterswijk CA
FAU - Karperien, Marcel
AU  - Karperien M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130529
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
SB  - IM
MH  - Antibodies, Neutralizing/immunology
MH  - Bone Marrow Cells/metabolism
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chondrocytes/*metabolism
MH  - Coculture Techniques
MH  - Fibroblast Growth Factor 1/genetics/immunology/*metabolism
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Humans
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Osteoarthritis/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Fibroblast Growth Factor/antagonists & inhibitors
MH  - Up-Regulation
PMC - PMC3749707
EDAT- 2013/04/06 06:00
MHDA- 2014/04/01 06:00
PMCR- 2014/09/01
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 10.1089/scd.2013.0118 [pii]
AID - 10.1089/scd.2013.0118 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2013 Sep 1;22(17):2356-67. doi: 10.1089/scd.2013.0118. Epub 2013 
      May 29.