PMID- 23557548
OWN - NLM
STAT- MEDLINE
DCOM- 20131211
LR  - 20161125
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 126
IP  - 7
DP  - 2013 Apr
TI  - Mycophenolate mofetil affects monocyte Toll-like receptor 4 signaling during 
      mouse renal ischemia/reperfusion injury.
PG  - 1224-9
AB  - BACKGROUND: Mycophenolate mofetil (MMF) has been used to prevent transplant 
      rejection for many years and has been shown to have protective effects against 
      renal failure. The objective was to investigate the effect of MMF on monocyte 
      Toll-like receptor 4 (TLR4) signaling in the early stages of renal 
      ischemia/reperfusion injury (IRI) of mice. METHODS: Sixty BALB/C mice were 
      randomly divided into two groups: an IRI group, in which renal IRI was induced by 
      clamping the renal pedicles for 45 minutes, and an MMF group, in which MMF was 
      given (40 mgxkg(-1)xd(-1), intraperitoneally) from 2 days before renal IRI. The 
      plasma creatinine level and renal tissue damage of each group mice were observed 
      6, 12, 24, and 48 hours after reperfusion. The concentration of plasma 
      high-mobility group box 1 (HMGB-1) (TLR4 ligand), interleukin 6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), and tumor necrosis factor alpha (TNF-alpha) and the 
      expression of TLR-4 on monocytes were determined. RESULTS: The plasma creatinine 
      concentration in the MMF group was lower compared to the IRI group (after 
      reperfusion of 6, 12, 24, or 48 hours, P < 0.05). Pathological analysis showed 
      that the renal damage was slighter, TLR-4 expression was reduced (after 
      reperfusion of 6, 12, 24, or 48 hours, P < 0.05), and the concentration of 
      cytokines in the plasma was lower (P < 0.05) in the MMF group. No differences in 
      the concentrations of HMGB-1 were observed (P > 0.05). CONCLUSION: Monocyte TLR4 
      signaling is important in the early stage of kidney IRI, but MMF can inhibit it 
      and improve renal function.
FAU - Zhang, Yan-Xia
AU  - Zhang YX
AD  - Department of Nephropathy, Affiliated Beijing Friendship Hospital, Capital 
      Medical University, Beijing 100050, China.
FAU - Zhang, Jian-Rong
AU  - Zhang JR
FAU - Wang, Zhi-Gang
AU  - Wang ZG
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - AYI8EX34EU (Creatinine)
RN  - HU9DX48N0T (Mycophenolic Acid)
SB  - IM
MH  - Acute Kidney Injury/blood/*drug therapy/*metabolism
MH  - Animals
MH  - Chemokine CCL2/genetics/metabolism
MH  - Creatinine/blood
MH  - Cytokines/blood
MH  - HMGB1 Protein/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/drug effects/metabolism
MH  - Mycophenolic Acid/*analogs & derivatives/therapeutic use
MH  - Random Allocation
MH  - Reperfusion Injury/blood/*drug therapy/*metabolism
MH  - Signal Transduction/drug effects/genetics
MH  - Toll-Like Receptor 4/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2013/04/06 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2013 Apr;126(7):1224-9.