PMID- 23557759
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20220105
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 100
IP  - 1
DP  - 2013 Jul
TI  - Estrogen receptor beta agonist diarylpropionitrile inhibits 
      lipopolysaccharide-induced regulated on activation normal T cell expressed and 
      secreted (RANTES) production in macrophages by repressing nuclear factor kappaB 
      activation.
PG  - 234-40
LID - S0015-0282(13)00398-1 [pii]
LID - 10.1016/j.fertnstert.2013.02.052 [doi]
AB  - OBJECTIVE: To investigate the effect of the estrogen receptor-beta (ERbeta) agonist 
      diarylpropionitrile (DPN) on lipopolysaccharide (LPS)-induced regulated on 
      activation normal T cell expressed and secreted (RANTES) production in 
      macrophages and the possible mechanisms. DESIGN: Cellular and molecular biology 
      experimental study. SETTING: University-based research laboratory. PATIENT(S): 
      None. INTERVENTION(S): ERbeta mRNA and protein expression determined in murine 
      macrophage cell line RAW264.7 cells using reverse-transcription polymerase chain 
      reaction and Western blot analysis; RANTES production detected by ELISA in 
      LPS-stimulated RAW264.7 cells and ERbeta knockdown RAW264.7 cells after the addition 
      of DPN, phosphorylation of p65 and IkappaB degradation detected by Western blot 
      analysis; and nuclear accumulation of p65 visualized using immunofluorescence. 
      MAIN OUTCOME MEASURE(S): LPS-induced RANTES production and phosphorylation of p65 
      and IkappaB. RESULT(S): ERbeta was expressed in RAW264.7 cells, and DPN statistically 
      significantly decreased LPS-induced RANTES production in RAW264.7 cells. Small 
      interfering RNA targeting the ERbeta gene inhibited the effect of DPN on RANTES 
      production. In addition, DPN inhibited nuclear translocation and phosphorylation 
      of p65 by inhibiting IkappaB degradation and thus prohibited the activation of 
      nuclear factor kappaB (NF-kappaB). CONCLUSION(S): Diarylpropionitrile down-regulates 
      LPS-induced RANTES production via ERbeta. This effect of DPN is likely due to 
      repression of nuclear factor kappaB activation.
CI  - Copyright (c) 2013 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Huang, Shi-ying
AU  - Huang SY
AD  - Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, 
      People's Republic of China.
FAU - Xin, Hong
AU  - Xin H
FAU - Sun, Jing
AU  - Sun J
FAU - Li, Rui
AU  - Li R
FAU - Zhang, Xue-mei
AU  - Zhang XM
FAU - Zhao, Dong
AU  - Zhao D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130401
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (2,3-bis(4-hydroxyphenyl)-propionitrile)
RN  - 0 (Ccl5 protein, mouse)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitriles)
RN  - 0 (Propionates)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL5/*biosynthesis/metabolism
MH  - Estrogen Receptor beta/*agonists/physiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Lipopolysaccharides/antagonists & inhibitors/toxicity
MH  - Macrophages/drug effects/*metabolism
MH  - Mice
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Nitriles/*pharmacology
MH  - Propionates/*pharmacology
MH  - T-Lymphocytes/drug effects/*metabolism
EDAT- 2013/04/06 06:00
MHDA- 2013/09/05 06:00
CRDT- 2013/04/06 06:00
PHST- 2012/01/03 00:00 [received]
PHST- 2013/02/28 00:00 [revised]
PHST- 2013/02/28 00:00 [accepted]
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
AID - S0015-0282(13)00398-1 [pii]
AID - 10.1016/j.fertnstert.2013.02.052 [doi]
PST - ppublish
SO  - Fertil Steril. 2013 Jul;100(1):234-40. doi: 10.1016/j.fertnstert.2013.02.052. 
      Epub 2013 Apr 1.