PMID- 23557933
OWN - NLM
STAT- MEDLINE
DCOM- 20131108
LR  - 20220408
IS  - 1096-1186 (Electronic)
IS  - 1043-6618 (Linking)
VI  - 72
DP  - 2013 Jun
TI  - One-year supplementation with a grape extract containing resveratrol modulates 
      inflammatory-related microRNAs and cytokines expression in peripheral blood 
      mononuclear cells of type 2 diabetes and hypertensive patients with coronary 
      artery disease.
PG  - 69-82
LID - S1043-6618(13)00060-1 [pii]
LID - 10.1016/j.phrs.2013.03.011 [doi]
AB  - Numerous studies have shown that resveratrol (RES) exerts anti-inflammatory 
      effects but human trials evidencing these effects in vivo are limited. 
      Furthermore, the molecular mechanisms triggered in humans following the oral 
      intake of RES are not yet understood. Therefore, the purpose of this study was to 
      investigate the molecular changes in peripheral blood mononuclear cells (PBMCs) 
      associated to the one-year daily intake of a RES enriched (8 mg) grape extract 
      (GE-RES) in hypertensive male patients with type 2 diabetes mellitus (T2DM). We 
      used microarrays and RT-PCR to analyze expression changes in genes and microRNAs 
      (miRs) involved in the inflammatory response modulated by the consumption of 
      GE-RES in comparison to a placebo and GE lacking RES. We also examined the 
      changes in several serobiochemical variables, inflammatory and fibrinolytic 
      markers. Our results showed that supplementation with GE or GE-RES did not affect 
      body weight, blood pressure, glucose, HbA1c or lipids, beyond the values 
      regulated by gold standard medication in these patients. We did not find either 
      any significant change on serum inflammatory markers except for a significant 
      reduction of ALP and IL-6 levels. The expression of the pro-inflammatory 
      cytokines CCL3, IL-1beta and TNF-alpha was significantly reduced and that of the 
      transcriptional repressor LRRFIP-1 increased in PBMCs from patients taking the 
      GE-RES extract. Also, a group of miRs involved in the regulation of the 
      inflammatory response: miR-21, miR-181b, miR-663, miR-30c2, miR-155 and miR-34a 
      were found to be highly correlated and altered in the group consuming the GE-RES 
      for 12 months. Our results provide preliminary evidence that long-term 
      supplementation with a grape extract containing RES downregulates the expression 
      of key pro-inflammatory cytokines with the involvement of inflammation-related 
      miRs in circulating immune cells of T2DM hypertensive medicated patients and 
      support a beneficial immunomodulatory effect in these patients.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Tome-Carneiro, Joao
AU  - Tome-Carneiro J
AD  - Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, 
      30100 Campus de Espinardo, Murcia, Spain.
FAU - Larrosa, Mar
AU  - Larrosa M
FAU - Yanez-Gascon, Maria J
AU  - Yanez-Gascon MJ
FAU - Davalos, Alberto
AU  - Davalos A
FAU - Gil-Zamorano, Judit
AU  - Gil-Zamorano J
FAU - Gonzalvez, Manuel
AU  - Gonzalvez M
FAU - Garcia-Almagro, Francisco J
AU  - Garcia-Almagro FJ
FAU - Ruiz Ros, Jose A
AU  - Ruiz Ros JA
FAU - Tomas-Barberan, Francisco A
AU  - Tomas-Barberan FA
FAU - Espin, Juan Carlos
AU  - Espin JC
FAU - Garcia-Conesa, Maria-Teresa
AU  - Garcia-Conesa MT
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130401
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (Plant Extracts)
RN  - 0 (Stilbenes)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Aged
MH  - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
MH  - Coronary Artery Disease/complications/*diet therapy/genetics/immunology
MH  - Cytokines/*genetics/immunology
MH  - Diabetes Mellitus, Type 2/complications/*diet therapy/genetics/immunology
MH  - Dietary Supplements/analysis
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Hypertension/complications/*diet therapy/genetics/immunology
MH  - Inflammation/drug therapy/genetics/immunology
MH  - Leukocytes, Mononuclear/*drug effects/immunology/metabolism
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Models, Molecular
MH  - Plant Extracts/therapeutic use
MH  - Resveratrol
MH  - Stilbenes/*therapeutic use
MH  - Transcriptome/drug effects
MH  - Vitis/chemistry
EDAT- 2013/04/06 06:00
MHDA- 2013/11/10 06:00
CRDT- 2013/04/06 06:00
PHST- 2013/02/20 00:00 [received]
PHST- 2013/03/25 00:00 [revised]
PHST- 2013/03/25 00:00 [accepted]
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/11/10 06:00 [medline]
AID - S1043-6618(13)00060-1 [pii]
AID - 10.1016/j.phrs.2013.03.011 [doi]
PST - ppublish
SO  - Pharmacol Res. 2013 Jun;72:69-82. doi: 10.1016/j.phrs.2013.03.011. Epub 2013 Apr 
      1.