PMID- 23558047
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1289
DP  - 2013 May 10
TI  - Improvement of a gas chromatographic method for the analysis of iminosugars and 
      other bioactive carbohydrates.
PG  - 145-8
LID - S0021-9673(13)00451-2 [pii]
LID - 10.1016/j.chroma.2013.03.013 [doi]
AB  - The analysis of derivatised iminosugars and other bioactive low molecular weight 
      carbohydrates present at low concentrations in different vegetable extracts 
      (hyacinth, mulberry and buckwheat) required the improvement of a previously 
      developed gas chromatographic method. Among the different parameters optimized, 
      the temperature of the injection port, evaluated for the first time, resulted to 
      be the most important. Thus, 240  degrees C was chosen as a tradeoff to achieve the 
      required volatility, to avoid degradation and to provide symmetric peaks for 
      bioactive carbohydrates highly retained such as glycosyl inositols. GC-MS 
      operating under selective ion monitoring (SIM) acquisition mode and GC-FID 
      provided limits of detection and quantitation for the target compounds of 0.2 and 
      0.7 ng g(-1) and of 1 and 3 ng g(-1) on average, respectively. Therefore, both 
      methodologies could be considered appropriate to extend the range of quantitation 
      of these bioactives.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Rodriguez-Sanchez, S
AU  - Rodriguez-Sanchez S
AD  - Instituto de Quimica Organica General (CSIC), Juan de la Cierva 3, 28006 Madrid, 
      Spain.
FAU - Soria, A C
AU  - Soria AC
FAU - Ruiz-Matute, A I
AU  - Ruiz-Matute AI
FAU - Sanz, M L
AU  - Sanz ML
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130316
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Carbohydrates)
RN  - 0 (Imino Sugars)
SB  - IM
MH  - Carbohydrates/*analysis
MH  - Chromatography, Gas/*methods
MH  - Fruit/*chemistry
MH  - Imino Sugars/*analysis
MH  - Vegetables/*chemistry
EDAT- 2013/04/06 06:00
MHDA- 2013/09/24 06:00
CRDT- 2013/04/06 06:00
PHST- 2012/09/25 00:00 [received]
PHST- 2013/02/15 00:00 [revised]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2013/04/06 06:00 [entrez]
PHST- 2013/04/06 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
AID - S0021-9673(13)00451-2 [pii]
AID - 10.1016/j.chroma.2013.03.013 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 May 10;1289:145-8. doi: 10.1016/j.chroma.2013.03.013. Epub 
      2013 Mar 16.